Reaction mass of pentan-1-ol and 2-methylbutan-1-ol

Repeated dose toxicity: oral

Administrative data

Endpoint:

sub-chronic toxicity: oral

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

A read-across justification is provided in the IUCLID chapter "assessment reports"

Data source

Reference

Reference Type:

publication

Title:

Effects of several alcohols on glucosidase activities in rat liver and brain

Author:

San-Juan I et al.

Year:

1992

Bibliographic source:

Comp. Biochem. Physiol. Vol. 101B, No. 1/2, pp. 41-47

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Analysis of glycosidases from liver and brain of alcohol-treated rats and their kinetics

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Pentan-1-ol

EC Number:

200-752-1

EC Name:

Pentan-1-ol

Cas Number:

71-41-0

Molecular formula:

C5H12O

IUPAC Name:

pentan-1-ol

Details on test material:

- Name of test material (as cited in study report): amyl alcohol
- Source: Mallinckrodt

Test animals

Species:

rat

Strain:

Wistar

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS

- Age at study initiation: 35 days
- Weight at study initiation: mean 75 g
- Diet (e.g. ad libitum): A-04 rat chow from Panlab (Barcelona, Spain), containing 17% protein, 3% lipids and 59% carbohydrates; ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 22
- Photoperiod (hrs dark / hrs light): 12 / 12

Administration / exposure

Route of administration:

oral: drinking water

Vehicle:

water

Analytical verification of doses or concentrations:

no

Duration of treatment / exposure:

50 day

Frequency of treatment:

continuously, ad libitum

Doses / concentrations

Remarks:

Doses / Concentrations:
1 -4% for 8 days , followed by an additional period of 40-50 days using a 4% aqueous alcohol solution
Basis:
nominal in water

No. of animals per sex per dose:

no data

Control animals:

yes, concurrent vehicle

Details on study design:

The alcohols were given progressively at 1-4% for 8 days (adaptation period), followed by an additional period of 40-50 days using a 4% aqueous alcohol solution. During the same period of time, control rats, belonging to the same litter as the alcohol-treated rats, drank only water.

Results and discussion

Effect levels

Dose descriptor:

NOAEL

Remarks on result:

not determinable

Remarks:

no NOAEL identified

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

The only effects on glycosidases seen after treatment with amyl alcohol was an significant increase in the activity of beta-D-glucuronidase from brain (0.09 +/-0.02 mU/mg protein; control 0.08 +/-0.01 mU/mg protein).