Ethyl 2,6,6-trimethyl-4-oxocyclohex-2-ene-1-carboxylate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 April to 5 August 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 2,6,6-trimethyl-4-oxocyclohex-2-ene-1-carboxylate

EC Number:

245-410-2

EC Name:

Ethyl 2,6,6-trimethyl-4-oxocyclohex-2-ene-1-carboxylate

Cas Number:

23068-96-4

Molecular formula:

C12H18O3

IUPAC Name:

ethyl 2,6,6-trimethyl-4-oxocyclohex-2-ene-1-carboxylate

Specific details on test material used for the study:

Name (as stated in the report): Carbethoxy Isophorone
Batch: SC00015362
Appearance: Yellow liquid
Expiration date: August 14, 2017

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage was used. The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant ans resuspending in mineral medium) and kept aerobic until being used on the same day.

Duration of test (contact time):

66 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

30 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

100 mg/L

Based on:

other: reference substance

Reference substance

Reference substance:

other: sodium benzoate

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

78

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Carbethoxy Isophorone undergoes 78% biodegradation after 28 days in the tests conditions. The 10 -day window criterion is also fulfilled (11% biodegradation on day 11 and 67% on day 21).
Carbethoxy Isophorone did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore consider the be non toxic to the inoculum at the test concentration. Thus, Carbethoxy Isophorone should be regarded as readily biodegradable according to this test.

Executive summary:

The Ready biodegradability of Carbethoxy Isophorone was determined by the Manometric Respirometry test. Carbethoxy Isophorone undergoes 78% biodegradation after 28 days in the tests conditions. The 10 -day window criterion is also fulfilled (11% biodegradation on day 11 and 67% on day 21).

Carbethoxy Isophorone did not inhibit the intrinsic respiration of the inoculum at the test concentration and was therefore consider the be non toxic to the inoculum at the test concentration. Thus, Carbethoxy Isophorone should be regarded as readily biodegradable according to this test.