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Diss Factsheets
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EC number: 432-070-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: no melting point/range (Decomposition at temperatures > 200 °C)
- Boiling point: no boiling point (decomposition before boiling)
- Vapour pressure: <1 * 10-12 hPa at 25°C (calculation)
- Water solubility: 125.76 g/L at 20°C
- log Pow: <-2
- pKa: = 7,00+/-0,04 - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 50, 100, 1250, 2500, 500, 10000 mg/L,
- Vehicle:
- no
- Details on test solutions:
- The Test article is a well soluble powder with strongly reduginc properties. This would lead to total oxygen depletion in the test flasks and consequent growth inhibition. According to former tests with the test item, the test article was solved in deionized water and aerated with cleaned compressed air for 24 hours at 20°C until complete oxidation. The test concentrations were produced by diluting the stock solution with test water.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Scenedesmus subspicatus SAG 86.81, Chlorophyceae, Chlorococales
-stock culture/method of cultivation: on a sterile sloped agar tube (OECD mdium, 0.8% Agar), transfer to a new agar tube every 2 month
-pre-culture: Incubation on a shaker in 250 mL Erlenmeyer flasks, sterile, ca 72 h, 25°C, 100-130 rpm, 10000 lux, permanent illumination
ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no observation period given in the study
- Hardness:
- no hardness indicated in the study
- Test temperature:
- 25° C +/- 2°C, climated room
- pH:
- 8
- Dissolved oxygen:
- no data available
- Salinity:
- see "details on test conditions": Solution 1, 2, 3 and 4
- Nominal and measured concentrations:
- nominal concentrations: 50 - 100 - 1250 - 2500 - 5000 - 10000 - Control [mg/L]
measured concentrations: no data available - Details on test conditions:
- TEST SYSTEM
- Initial cells density: approx. 10000 cells/mL
- Control end cells density: 1055107 cells/mL (average of control 1,2 and 3 after 72 h)
TEST MEDIUM / WATER PARAMETERS
The Test article is a well soluble powder with strongly reducing properties. This would lead to total oxygen depletion in the test flasks and consequent growth inhibition. According to former tests with Bruggolite FF6 the test article was solved in deionized water and aerated with cleaned compressedair for 24 hours at 20°C until complete oxidation. The test concentrations were produced by diluting the stock solution with test water.
preparation of:
Solution 1 (according to OECD 201):
- NH4Cl (1.5 g/L)
- MgCl2 x 6 H2P (1.2 g/L)
- CaCl2 x 2 H2O (1.8 g/L)
- MgSo4 x 7 H20 (1.5 g/L)
- KH2Po4 (0.16 g/L)
Solution 2:
- FeCl3 x 6 H2O (0.08 g/L)
- Na2 EDTA x 2 hso (0.1 g/L)
Solution 3:
- H3BO3 (0.185 g/L)
- MnCl2 x 4 H2= (0.415 g/L)
- Na2MoO4 x 2 H2= (0.0007 g/L)
- Zncl2 (30 mL stock solution), stock solution with C= 100 mg/L
- CoCl2 x 6 H2O (15 mL stock solution), stock solution with C= 100 mg/L
-CuCl2 x 2H2O (100 µL stock solution), stock solution with C= 100 mg/L
Solution 4:
- NaHCo3 (50.0 g/L)
the medium is made up with water an is autoclaved at 121 °C for 15 minutes. Solution 4 is added by sterile filtration (0.45 µm pore size).
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment of pH
- Light intensity and quality/Illumination: ca. 10 000 Lux, permanent illumination
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chambers (Fuchs-Rosenthal: chamber factor 80000, Neubauer: chamber factor 4000000)
- Spectrophometer 662, Methrom
- measurement of the optical density at 520 nm ath the times 24, 48 and 72 h and calculation of the cell concentration (per mL). The average of the cell concentrations is plotted against the time to obtain the growth curve.
TEST CONCENTRATIONS
- Test concentrations: 50, 100, 1250, 2500, 5000, 10000 mg/L - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- other: EbC50
- Effect conc.:
- 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 315 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Test concentrations of 50 and 100 mg/L caused moderate inhibition of algae growth after 72 h. The test concentrations 1250, 2500, 5000 and 10000 mg/L resultet in total inhibition after 72 h.
- Results with reference substance (positive control):
- no reference substance (positive control) was tested.
- Reported statistics and error estimates:
- no data available
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item is not acute toxic to Algae.
- Executive summary:
the substance is not acute toxic to Algae.
EbC50 (72h)= 300 mg/l;
ErC50( 72h) = 315 mg/l
Reference
Description of key information
the substance is not acute toxic to Algae.
EbC50 (72h)= 300 mg/l;
ErC50( 72h) = 315 mg/l
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 300 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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