Reaction product of 3,9-dibromobenzanthrone condensed with 2 equivalents of 1-aminoanthraquinone, subsequently further condensed under oxidative conditions

Endpoint summary

Description of key information

Short-term toxicity data for freshwater organisms are available for the substance at three trophic levels: aquatic invertebrates, fish and algae. The toxicity of the substance to activated sludge was also determined in a 3-hours respiration inhibition test.

A GLP study was performed with the substance in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test) and EU Method C.1 (Acute Toxicity for Fish). Lethality was observed in the 50 mg/L group (3/7 fish) and in the 100 mg/L group (1/7 fish) but not in the other tested groups; no lethality occurred at 220 and 500 mg/L. The test concentrations were present as stable suspensions in test water. The substance concentration was determined in all tested concentration groups after 0, 48 and 96 hours (in the 500 mg/L group, only the value at study start was determined). The values determined show a slight decrease of the test concentrations over 96 hours but were all in a range of +/- 20 % of the theoretical value. The observed adverse effects to fish were not considered to be related to the concentration of the test substance. Therefore, it can be assumed, that the effects are not caused by the soluble fraction. Mechanical or other physical effects of the test were not reproducible. As no lethality occurred at 220 and 500 mg/L, the LC50 after 96 hours exceeds 500 mg/L.

 

The acute toxicity of the test material to Daphnia magna was determined in a GLP study performed in accordance with OECD Guideline 202 and EU Method C.2. The nominal concentrations tested were 58 mg/L, 100 mg/L and 180 mg/L, and a control without any additions. The control and all test concentrations did not cause any immobilization of the daphnids after 48 hours. The EC0 value is > 180 mg/L. The EC50 and EC100 values are clearly higher than 180 mg/L nominal. The test substance is not soluble in water and organic solvents therefore measured concentrations could not be determined. It is concluded that the substance is practically non-toxic to Daphnia magna.

In a GLP study conducted in accordance with OECD Guideline 201, Vat Black 25 was found to inhibit the growth of the freshwater green alga Pseudokirchneriella subcapitata after 72 hours with the following effect values (based on nominal test item loadings): The EL50-values with 95 % confidence intervals for inhibition of growth rate (ErL50) after 72 hours were 3.08 (2.92 – 3.36) mg/L, corresponding to 61.6 (58.5 – 67.2) % of the saturated solution, respectively. The EL50-values with 95 % confidence intervals for inhibition of yield (EyL50) after 72 hours were 1.88 (1.67 – 2.06) mg/L, corresponding to 37.5 (33.4 – 41.1) % of the saturated solution, respectively. The NOEL-values for inhibition of growth rate and yield after 72 hours were 1.25 mg/L (corresponding to 25.0 % of the saturated solution) and < 0.313 mg/L (corresponding to < 6.25 % of the saturated solution), respectively.

 

The toxicity of the substance to activated sludge was evaluated in a GLP study conducted in accordance with OECD Guideline No. 209. The substance concentrations tested were 64, 20 and 6.4 mg/L. The IC50 of the test material was determined to be > 64.0 mg/L.