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EC number: 203-008-4 | CAS number: 102-16-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from ABITEC study report.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Principles of method if other than guideline:
- Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Benzyl phenylacetate (CAS no. 102-16-9) according to OECD Guideline 201.
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Benzyl phenylacetate
- Molecular formula (if other than submission substance): C15H14O2
- Molecular weight (if other than submission substance): 226.2736 g/mol
- Smiles notation (if other than submission substance):c1(CC(OCc2ccccc2)=O)ccccc1
- InChI:1S/C15H14O2/c16-15(11-13-7-3-1-4-8-13)17-12-14-9-5-2-6-10-14/h1-10H,11-12H2
- Substance type: Organic
- Physical state: Liquid - Analytical monitoring:
- not specified
- Vehicle:
- yes
- Details on test solutions:
- The stock solution (100 mg/L) was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- ±1 hour
- Test temperature:
- 23±2°C
- pH:
- The sample at test concentration 100 mg/l : pH = 8.1 changed to pH = 7.7 during the test.
Control: 8.3 (changed to 7.9 during test)
control + acetone: pH = 8.3 changed to pH = 7.9 during the test - Nominal and measured concentrations:
- 0, 6.25, 12.5, 25, 50 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml Glass vessel
- Type (delete if not applicable): closed (with air permeable stopper)
- Sample volume: 15 ml
- Initial cells density: 5x10(3) cells/ml
- No. of vessels per concentration (replicates): 3
- Other:
Control solution: OECD growth medium without the sample, the coefficient of variation of average growth rate in replicates during the whole test period was 1.1% in control and 1.0% in control + acetone.
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Continuous
- Light intensity and quality: 6000-8000 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: microscope with counting chamber Cyrus I or electronic particle counter.
- Other: ErC50 was calculated using non-linear regression by the software Prism 4.0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7) was used as a reference substance for the study.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 34.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 29.4 - 40.4
- Results with reference substance (positive control):
- Results with reference substance valid
- EC50: 0.69 mg/L - Validity criteria fulfilled:
- not specified
- Conclusions:
- Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Benzyl phenylacetate to OECD Guideline 201. The median effective concentration (EC50) for the test substance Benzyl phenylacetate in Desmodesmus subspicatus was determined to be 34.5 mg/L on the basis of effects on growth rate in a 72 hour study.
- Executive summary:
Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Benzyl phenylacetate (CAS no. 102 -16 -9) to OECD Guideline 201. The stock solution (100 mg/L) was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Benzyl phenylacetate in Desmodesmus subspicatus was determined to be 34.5 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical Benzyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.
Reference
Description of key information
Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Benzyl phenylacetate (CAS no. 102 -16 -9) to OECD Guideline 201 (ABITEC study report, Sustainability Support Services (Europe) AB, 2017). The stock solution (100 mg/L) was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Benzyl phenylacetate in Desmodesmus subspicatus was determined to be 34.5 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical Benzyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 34.5 mg/L
Additional information
Experimental key studyfor the target chemicalBenzyl phenylacetate(CAS no. 102-16-9) which is supported further by experimental study for its closest structurally similar read across substance with logKow as the primary descriptor were reviewed for the toxicity to aquatic algae and cyanobacteria end point which are summarized as below:
In an experimental key study,freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance Benzyl phenylacetate (CAS no. 102 -16 -9) according to OECD Guideline 201(Alga, Growth Inhibition Test) (ABITEC study report, Sustainability Support Services (Europe) AB, 2017). The stock solution (100 mg/L) was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Benzyl phenylacetate in Desmodesmus subspicatus was determined to be 34.5 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical Benzyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.
In a supporting study of read across substance 2-Phenethyl phenylacetate (CAS no. 102-20-5), the effect of read across substance 2-Phenethyl phenylacetate, CAS No. 102-20-5 was studied on the growth of fresh water green alga Chlorella vulgaris (UERL Study report, Sustainability Support Services (Europe) AB, 2015). The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 2 mg/l, 3 mg/l, 4.5 mg/l, 6.75 mg/l, 10.13 mg/l and 15.19 mg/l were prepared using stock solution of the test substance using de-ionized water. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of test substance. EC50 calculated graphically through probit analysis was observed to be 13.89 mg/l. Thus, based on this value, test chemical 2-Phenethyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.
Thus, based on the overall reported results for target chemicalBenzyl phenylacetate (ABITEC study report, 2017) and for its read across substance (UERL study report, 2015), it can be concluded that the test substanceBenzyl phenylacetatecan be considered astoxic to aquatic organisms at environmentally relevant concentrations and can be considered to be classified inaquatic chronic category 3as per the CLP classification criteria.
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