Carbamic acid, (diaminophosphinyl)-, 1-methylethyl ester

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001-02-06 - 2001-03-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

isopropyl (diaminophosphoryl) carbamate

Cas Number:

298692-41-8

Molecular formula:

C4H12N3O3P

IUPAC Name:

isopropyl (diaminophosphoryl) carbamate

Details on test material:

- Analytical purity: > 95%
- Lot/batch No.: S-2

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

6 weeks old, nulliparous, non-pregnant,
mean body weight: males: 240g (n=3)
females: 204 g (n=6)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: suspension in 0.5 % solution of Tylose MH 1000 in deionised water

Doses:

200 mg/kg, 2000 mg/kg

No. of animals per sex per dose:

200 mg/kg: 3 female animals
2000 mg/kg: 3 female and 3 male animals

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical signs: once daily, body weight: day 0, 7, 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology,

Statistics:

Body weights and body weight gain
Calculation of group mean values and standard deviations
Comparism with historical control data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 200 - < 2 000 mg/kg bw

Mortality:

Animals were observed for mortality and morbidity continuously on the day of administration and once daily thereafter (in the morning).
Number of dead animals (200 mg/kg b.w.): 0
Number of deaad animals (2000 mg/kg b.w.): 2 females (67 %)

Clinical signs:

other: The animals were monitored for general clinical condition continuously on the day of the administration and once daily thereafter (in the morning). The following signs were given predominant consideration: changes in skin, fur, eyes and mucous membranes,

Gross pathology:

At the end of the observation period all surviving animals were killed by CO2 inhalation. All animals were examined externally. The cranial, thoracic and abdominal cavities were then opened and examined macroscopically. Punctae haemorrhages in the glandular stomach membrane, a reddish brown discoloured intestine content and many atelectatic areas in the lung were observed in one died female animal. The remaining animals showed only atelectatic areas in the lung.
Macroscopic pathological findings were not observed in the surviving animals.

Other findings:

non

Applicant's summary and conclusion

Interpretation of results:

harmful

Remarks:

Migrated information symbol of danger: Xn Criteria used for interpretation of results: EU

Conclusions:

The acute oral toxicity of N-(Isopropoxycarbonyl) phosphoric acid triamide was tested in Charles River Wistar rats. The test item was administred at the single dose of 2000 mg/kg body weight (b.w.) to 3 male and 3 female animals each and at the single dose of 200 mg/kg b.w. to 3 female animals by gavage.The mortality at 2000 mg/kg was 67% (females). At 200 mg/kg non of the animals died.

Executive summary:

The acute oral toxicity of N-(Isopropoxycarbonyl) phosphoric acid triamide was tested in Charles River Wistar rats. The test item was administered at a dose of 2000 mg/kg body weight (b.w.) to 3 male and 3 female animals each and at a dose of 200 mg/kg b.w. to 3 female animals by gavage.The mortality at 2000 mg/kg was 67% (females). At 200 mg/kg non of the animals died.