Formamide, N-[[(4-methylphenyl)sulfonyl]methyl]-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

yes

Remarks:

Since concentrations were below the detection limit at the end of exposure, values based upon initial concentration. Validity criterion for maintaining the conc over the test period is not fulfilled.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N-[[(4-methylphenyl)sulfonyl]methyl]formamide

EC Number:

700-490-2

Cas Number:

36635-56-0

Molecular formula:

C9H11NO3S

IUPAC Name:

N-[[(4-methylphenyl)sulfonyl]methyl]formamide

Details on test material:

- Name of test material (as cited in study report): CGA 224433
- Physical state: white solid
- Analytical purity: 100%
- Lot/batch No.: P.709027

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Common name: Scenedesmus subspicatus
- Strain: Cambridge 276/20
- Initial cell density: 1.27*1E4 cells/ml
- Preculture: 3 days under test conditions

Study design

Test type:

static

Water media type:

not specified

Total exposure duration:

72 h

Test conditions

Test temperature:

24 ± 2°C

Nominal and measured concentrations:

Actual concentration: 3.0, 8.8, 29, 55, 130, 240mg/ml

Details on test conditions:

TEST SYSTEM
- Test vessel: 100ml Erlenmeyer flasks stoppered with aluminium caps
- Type: closed
- Material, size, headspace, fill volume: 50ml test solution
- Aeration: none
- Initial cells density: 1.27*1E4 cells/ml
- No. of vessels per concentration (replicates): 3 replicates per test concentration
- No. of vessels per control (replicates): 6 replicates for blank

OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity and quality: approx 8000 lux (120µE/m2sec)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] TOA cell counter
- pH: at start and end of exposure

TEST CONCENTRATIONS
- Test concentrations: 18, 32, 58, 100, 180 and 320mg/ml

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

ca. 38 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

growth rate

Remarks on result:

other: none

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 3 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

growth rate

Remarks on result:

other: none

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC0

Effect conc.:

ca. 3 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

EC100

Effect conc.:

>= 240 mg/L

Nominal / measured:

meas. (initial)

Basis for effect:

growth rate

Details on results:

Calculations:
EC50 results were calculated according to BERKSON, JASA 48 (1953), 569-599
EC values graphically determined on gausso-logarithmic probability paper.
NOEC was calculated according to DUNNETT, C.W., JASA 50 (1955), 1096-1121, Biometrics 20 (1964), 482-491, and modified by MARCUS et al., Biometrika 63 (1976), 655-660.

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Remarks:

Since concentrations were below the detection limit at the end of exposure, values based upon initial concentration. Validity criterion for maintaining the conc over the test period is not fulfilled.

Conclusions:

Testing for acute toxicity of CGA 224433 to Green Algae showed evidence of reduction in growth and thus deemed harmful to green algae.

Executive summary:

Testing was carried out to determine the effects (if any) of CGA 224433 upon Green Algae.

It was evident from the testing that reduced growth did occur and that a classification of effects to the environment is required.

An EbC50 72 hr value was be calculated at 38 mg/ml and NOEbC 72 hr value of 3.0 mg/ml

This substance should therefore be classified as R52 harmful to the environment and R53 may cause long-term adverse effects in the aquatic environment according to EU criteria (DSD) and Acute Hazards Category: Chronic III according to GHS.