Tetrasodium 4-amino-5-hydroxy-3,6-bis[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Study period:

2021

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

The prediction has been carried out by means of QSAR prediction, using the following
model:
• “ISIDA Consensus – Ready Biodegradability (RB) classification model”. The
relative QMRF is available as attached file (“5.2.1 - EC 241-164-5 - Reactive
Black 5 - ISIDA_RB_QMRF.pdf”).

Data source

Reference

Reference Type:

other company data

Title:

Unnamed

Year:

2021

Materials and methods

Principles of method if other than guideline:

In-silico prediction (QSAR) has been used to cover the present endpoint. The aim of
this prediction is to contribute through a weight of evidence approach to the
assessment of the Ready Biodegradability in order to satisfy the REACH requirements
for the registration dossier using non-testing methods.

Test material

Test material information

Constituent 1

Reference substance name:

Tetrasodium 4-amino-5-hydroxy-3,6-bis[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate

EC Number:

241-164-5

EC Name:

Tetrasodium 4-amino-5-hydroxy-3,6-bis[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate

Cas Number:

17095-24-8

Molecular formula:

C26H25N5O19S6.4Na

IUPAC Name:

tetrasodium 4-amino-5-hydroxy-3,6-bis[(4-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2,7-disulfonate

Test material form:

solid: particulate/powder

Results and discussion

% Degradation

Remarks on result:

not readily biodegradable based on QSAR/QSPR prediction

Remarks:

Applied model predicts the test material as NON Readily Biodegradable. The degradation % of the test substance can be considered to be <60 %: this value corresponds to the threshold necessary to define a substance as readily biodegradable as defined by OECD 301c. The test material fulfils the model's applicability domain, satisfying the defined reliability criteria. Moreover, structural analogues with known experimental data have been found in the model‘s training set, supporting the outcome of the test material. Thus, the prediction can be considered adequate for data-gap filling for the present endpoint.

Any other information on results incl. tables

The test material QPRF for the ISIDA model is available as attached file ("5.2.1 - EC
241-164-5 - Reactive Black 5_ISIDA_QPRF.xlsx”).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable