7-methyl-3-methyleneocta-1,6-diene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

No data

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

Old study conducted similarly to OECD Guideline 402 with minor deviations: few data about test substance and test conditions, skin was abraded.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

Few data about test substance and test conditions, skin was abraded.

Principles of method if other than guideline:

Not applicable

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

7-methyl-3-methyleneocta-1,6-diene

EC Number:

204-622-5

EC Name:

7-methyl-3-methyleneocta-1,6-diene

Cas Number:

123-35-3

Molecular formula:

C10H16

IUPAC Name:

7-methyl-3-methylideneocta-1,6-diene

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 1.7 to 2.4 kg

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
The test substance was applied at 5 g/kg bw on clipped abraded abdominal skin. The animals were wrapped with binders of rubber dam, gauze and adhesive tap.
Folllowing exposure, the binders were removed and observations were made.

Duration of exposure:

24 hours

Doses:

5 g/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: Animals were observed frequently for mortality for 14 days. Body weights were recorded at pre and post-treatment.
- Necropsy of survivors performed: Yes

Statistics:

None

Results and discussion

Preliminary study:

Not applicable

Effect levels

Key result

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality was observed.

Clinical signs:

other: No evidence of systemic toxicity. Signs of irritation were noted (see table 1).

Gross pathology:

No abnormalities were noted upon gross necropsy.

Other findings:

None

Any other information on results incl. tables

Table 1: signs of dermal irritation in rabbits following a 24-h exposure with myrcene

Observation day	Erythema	Edema
1	++ (8) + (2)	++ (9) + (1)
2	++ (5) + (5)	++ (5) + (5)
3	++ (3) + (6)	++ (3) + (6)
4	++ (1) + (7)	++ (2) + (6)
5	+ (5)	+ (4)
6	-	+ (2)
7	-	-
8	-	-
9	-	-
10	-	-
11	-	-
12	-	-
13	-	-
14	-	-

+ : slight ; ++ : moderate ; number in parentheses = number of animals showing sign

Table 2: bodyweights before and after treatment

Animal number	Pre-treatment (kg)	Post-treatment (kg)
1	2.0	1.9
2	1.7	1.8
3	1.9	2.0
4	2.0	1.9
5	2.4	2.4
6	2.0	2.0
7	1.8	1.8
8	2.1	2.0
9	2.2	2.1
10	2.2	2.2

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute dermal LD50 of myrcene was greater than 5 g/kg bw.

Executive summary:

In an acute dermal toxicity study performed similarly to OECD guideline 402, a group of 10 New Zealand White rabbits received a single dermal dose of myrcene at 5 g/kg on clipped abraded abdominal skin in occlusive conditions for 24 h. All animals were observed for mortality and skin reactions for 14 days. No mortality was observed. No changes in bodyweight related to treatment could be observed. No signs of systemic toxicity were observed. All animals showed slight to moderate erythema and edema during the first days after exposure that were completely reversed after 7 days.No abormalities were noted upon gross necropsy. Therefore, the acute dermal LD50 was greater than 5 g/kg bw.