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EC number: 202-996-4 | CAS number: 102-01-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- methaemoglobinaemia
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented study report which meets basic scientific principles. Study was conducted prior to GLP implementation.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Oral application of 100mg/kg to two female cats. Hematological examinations were made before and after treatment.
- GLP compliance:
- no
- Remarks:
- prior to GLP implementation
- Type of method:
- in vivo
Test material
- Reference substance name:
- Acetoacetanilide
- EC Number:
- 202-996-4
- EC Name:
- Acetoacetanilide
- Cas Number:
- 102-01-2
- Molecular formula:
- C10H11NO2
- IUPAC Name:
- 3-oxo-N-phenylbutanamide
- Details on test material:
- - Name of test material (as cited in study report): Acetessiganilid TTR
Constituent 1
Test animals
- Species:
- cat
- Strain:
- not specified
- Sex:
- female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: mucous starch (2%)
- Details on exposure:
- - single oral administration (gavage)
- 10% concentration of Acetessiganilid TTR in mucous starch (2%)
- dose level: 100 mg/kg bw
- examination before and 1, 3, 7, 24, 48, 72, and 96h, and 7, 14, 28 d post application. - Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- - Single application by gavage
- examination before and 1, 3, 7, 24, 48, 72, and 96h, and 7, 14, 28 d post application. - Frequency of treatment:
- Once
- Post exposure period:
- 28 d (blood sampling: 1h, 3h, 7h, 24h, 48h, 72h, 96h, 7d, 14d, 28d post application)
Doses / concentrations
- Remarks:
- Doses / Concentrations:
100 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 2 females
Examinations
- Examinations:
- Haematology: Haemoglobin, erythrocyte count, leucocyte count, haematocrit, haemogram, MetHb-formation, Heinz bodies
Results and discussion
- Details on results:
- Immediately after application both cats experienced increased salivation, 1hr p.a. an increased breathing frequency was observed as well as signs of cyanosis and vertigo. One cat vomited 1hr and 4.5hrs p.a.. Body weight gain was slightly reduced in both cats.
Application of 100mg/kg bw of Acetoacetanilide TTR caused formation of metheamoglobine and Ehrlich-Heinz-bodies.
Any other information on results incl. tables
|
|
Cat no. 1 |
Cat no. 2 |
|||||
Dose (mg/kg bw) |
Time post application |
MetHb (%)
|
Hb (%) |
Heinz bodies (%) |
MetHb (%)
|
Hb (%) |
Heinz bodies (%) |
|
100 |
control |
0 |
14.0 |
0.5 |
0 |
12.3 |
3.5 |
|
1h |
5.6 |
15.1 |
1.5 |
7.7 |
13.4 |
7.5 |
||
3h |
8.9 |
14.3 |
1.5 |
9.7 |
12.8 |
7.5 |
||
7h |
7.9 |
13.4 |
49.0 |
9.6 |
12.5 |
54.5 |
||
24h |
0.7 |
14.3 |
90.5 |
1.6 |
14.1 |
93.5 |
||
48h |
0.9 |
16.1 |
50.5 |
0.6 |
14.8 |
91.0 |
||
72h |
0.6 |
15.2 |
73.0 |
0.7 |
13.1 |
85.5 |
||
96h |
0.9 |
15.8 |
71.5 |
0 |
13.7 |
86.0 |
||
7d |
0.6 |
13.5 |
56.5 |
0.3 |
13.1 |
73.0 |
||
14d |
|
13.6 |
56.5 |
|
13.0 |
46.0 |
||
21d |
|
14.4 |
7.5 |
|
13.3 |
7.0 |
||
28d |
|
13.6 |
3.5 |
|
13.5 |
1.0 |
Applicant's summary and conclusion
- Conclusions:
- Data show that the test item causes MetHb and Heinz body formation. Data support the conclusion that MetHb related lethality may occur at doses higher than 100 mg/kg bw.
With respect to MetHb forming substances cats are known to react with higher sensitivity than humans. Based on the expected lethal dose of >100 mg/kg bw for the cat, the human acute toxic dose is estimated to be not higher than 2000 mg/kg bw. - Executive summary:
In order to assess the methaemoglobin-forming potential of Acetessiganilid TTR 2 cats received single oral doses of 100 mg/kg bw. Hematological examinations were made before and after treatment.
Immediately following application both cats experienced increased salivation, 1hr p.a. an increased breathing frequency was observed as well as signs of cyanosis and vertigo. One cat vomited 1hr and 4.5hrs p.a.. Body weight gain was slightly reduced in both cats. Application of 100mg/kg bw of Acetoacetanilide TTR caused formation of metheamoglobine and Ehrlich-Heinz-Bodies.
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