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Diss Factsheets

Environmental fate & pathways

Biodegradation in water and sediment: simulation tests

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Administrative data

Endpoint:
biodegradation in water and sediment: simulation tests
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Enzymatic degradation and assimilation of condensed phosphates by green algae
Author:
Davis EM and Wilcomb MJ
Year:
1967
Bibliographic source:
Water research 1:335-350

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The hydrolysis rates of condensed phosphates in domestic sewage with blue-green algae were determined using static runs of one month's duration and flow through studies of 1.5 days duration.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Pentasodium triphosphate
EC Number:
231-838-7
EC Name:
Pentasodium triphosphate
Cas Number:
7758-29-4
Molecular formula:
Na5P3O10 H5-xP3O10Nax (where x is approximately 5) 6H2O.Na5P3O10
IUPAC Name:
Pentasodium triphosphate
Details on test material:
- Name of test material (as cited in study report): Sodium tripolyphosphate (STPP)
Tetrapotassium pyrophosphate
Sodium hexametaphosphate
Radiolabelling:
no

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: Modified synthetic sewage medium
Duration of test (contact time):
36 h

Results and discussion

Any other information on results incl. tables

Examination of the results of the enzymatic hydrolytic activity of Scenedesmus spp shows what appears to be hydrolytic activity on the part of S. obliquus over that of S. quadricauda but it should be noted that the growth of S. obliquus was nearly double that of S. quadricauda at 30 days.

For the amount of growth of Ulothrix fimbriata surprisingly little phosphorus was assimilated by the organism. The hydrolytic activity was greater than for any of the other species.

Analyses of condensed phosphates added to column, column contents at t=0, column contents at t=36h and expected values of contents

Algal sp

P form added

Stock analysis (mg/l P)

column content at t=0 (mg/l P)

column content at t=36h (mg/l P)

Expected values (mg/l P)

 

 

ortho

condensed

ortho

condensed

ortho

condensed

ortho

condensed

Chlorella pyrenoidosa

STPP

0.0

6.20

1.70

2.02

0.59

2.42

0.05

6.09

TKPP

0.08

4.75

1.95

0.63

1.67

0.97

0.13

4.64

SHMP

0.41

5.79

2.22

1.70

1.15

1.75

0.46

5.68

C. vulgaris

STPP

0.05

4.90

3.00

0.01

0.78

2.22

0.13

4.77

TKPP

0.39

4.31

1.53

1.08

1.63

2.86

0.42

4.22

SHMP

0.13

5.87

0.85

0.98

0.46

1.50

0.15

5.74

Scenedesmus obliquus

STPP

0.13

2.87

2.61

3.74

1.51

1.36

0.20

2.89

TKPP

0.13

3.00

3.13

0.13

2.45

0.60

0.21

2.92

SHMP

0.00

4.17

3.00

0.00

1.37

1.76

0.08

4.06

S. quadricauda

STPP

0.03

6.56

1.70

1.04

0.78

1.90

0.08

9.41

TKPP

0.13

4.35

2.48

0.26

1.24

1.63

0.19

4.24

SHMP

0.06

3.73

1.37

0.00

1.37

1.50

0.09

3.63

Ulothrix fimbriata

STPP

0.13

3.59

5.49

0.19

2.22

0.83

0.27

3.50

TKPP

0.08

4.79

2.12

1.29

1.87

1.67

0.13

4.70

SHMP

0.13

6.07

3.85

1.73

1.39

1.48

0.23

5.95

Control

STPP

0.06

6.14

6.00

0.38

0.18

5.72

0.22

5.98

TKPP

0.04

4.83

4.25

0.36

0.09

4.57

0.15

4.71

SHMP

0.03

4.28

4.49

0.10

0.12

3.95

0.15

4.17

STPP

0.02

5.04

4.20

0.09

0.11

4.80

0.13

4.91

TKPP

0.03

4.83

4.52

0.10

0.05

4.49

0.11

4.65

SHMP

0.03

5.11

4.55

0.39

0.09

4.61

0.14

4.83

Rate constants for total phosphorus used by algae in column during 36h (1.5day) runs

Algal sp

P Form added

k*

% total P used**

Chlorella pyrenoidosa

STPP

-0.450

50.9

TKPP

-0.392

44.6

SHMP

-0.421

46.8

C. vulgaris

STPP

-0.325

38.7

TKPP

-0.006

1.0

SHMP

-0.732

66.7

Scenedesmus obliquus

STPP

-0.004

7.0

TKPP

-0.013

2.5

SHMP

-0.185

26.3

S. quadricauda

STPP

-0.588

58.6

TKPP

-0.288

35.2

SHMP

-0.169

22.7

Ulothrix fimbriata

STPP

-0.13

19.1

TKPP

-0.204

26.8

SHMP

-0.509

53.6

* in N0/N = kt units/day

**% total phosphorus taken from stock medium in 36h

Applicant's summary and conclusion