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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Literature data

Data source

Reference
Reference Type:
publication
Title:
The relative sensitivity of diatoms, snails, and fish to twenty common constituents of industrial wastes
Author:
Patrick Ruth, Cairns John and Arthur Scheier
Year:
1968
Bibliographic source:
The Progressive Fish-Culturist. 30(3):137- 140.

Materials and methods

Principles of method if other than guideline:
120 hours test, based on Patrick, 1964;Cairns, Scheier, and Hess, 1964. Essentially the diatoms were cultured in synthetic dilution water under controlled conditions. Tests were made by putting a standard inoculum of cells in each of the flasks used for the experiments and comparing the number of cells produced at the end of a 5-day growth period.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium sulphate
EC Number:
231-820-9
EC Name:
Sodium sulphate
Cas Number:
7757-82-6
Molecular formula:
H2O4S.2Na
IUPAC Name:
disodium sulfate

Test organisms

Test organisms (species):
Nitscheria linearis
Details on test organisms:
TEST ORGANISM
- Group: diatom
- Habitat: widely distributed in the unpolluted soft fresh waters of the United States as well as in other parts of the world.

ACCLIMATION
- Culturing media and conditions: diatoms were cultured in synthetic dilution water under controlled conditions.

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
5 d

Test conditions

Details on test conditions:
TEST SYSTEM
- Test vessel: 150-milliliter Erlenmeyer flasks
- Initial cells: tests were made by putting a standard inoculum of cells in each of the flasks used.

TEST MEDIUM / WATER PARAMETERS
Soft diluition water
- Ca(NO3)2 4H2O: 0.076 g/L
- Na2 SiO3 9H20: 0.177 g/L
- KCl: 0.020 g/L
- MgSO4 7H20: 0.40 g/L
- CaCO3: 0.10 g/L
- NaHCO3: 0.40 g/L
- K2HPO4: 0.004 g/L
- Ferric citrate: 0.0004 g/L

Results and discussion

Effect concentrations
Duration:
120 h
Dose descriptor:
EC50
Effect conc.:
ca. 1 900 mg/L
Nominal / measured:
nominal
Basis for effect:
cell number

Applicant's summary and conclusion

Conclusions:
Nitscheria linearis LD50 at 120 -h = 1900 ppm
Executive summary:

120 hours test, based on Patrick (1964) and Cairns et al. (1964) method, was conducted on Nitzschia linearis W, diatom. Essentially the diatoms were cultured in synthetic dilution water under controlled conditions. Tests were made by putting a standard inoculum of cells in each of the flasks used for the experiments and comparing the number of cells produced at the end of a 5-day growth period.

Result

Nitscheria linearis LD50 at 120 -h = 1900 ppm