Allyl heptanoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2022-06-15 to 2022-07-04

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

2019

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Each test concentration, the control and the solvent control were analytically verified once before the day of introduction of the fish and daily after the start of the exposure.

Vehicle:

yes

Remarks:

DMF was used as solvent with a loading rate of 0.1 mL/L. The solvent concentration was the same in all concentration levels and the solvent control (0.100 mL/L).

Details on test solutions:

Stock solution preparation:
A stock solution of 2000 mg/L was prepared in DMF. An appropriate volume of the test item was pipetted into a glass flask and filled up with the solvent. Density was taken into account. The solution was agitated until it is visually clear dissolved.
Further stock solutions were prepared by dilution with DMF. The stock solution was prepared in appropriate intervals of maximum 7 days.
The stock solutions were diluted considering the flow rate of the system. Test solutions flew through the test vessels for 14 days prior to the start of the exposure until measured concentrations of the test item show no trend of increasing or decreasing.

- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMF
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): 0.1 mL/L for all concentration levels and the solvent control
- Test concentration separation factor: 2.2

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Source: All fish used in the test were gained at Noack Laboratorien GmbH from a single brood stock (supplier: Umweltbundesamt,
Schichauweg 58, D-12307 Berlin)

HOLDING
Holding was performed at the test facility at 23 ± 2 °C and diffuse light (540 – 1000 Lux, photoperiod of 12 to 16 h light / 12 to 8 h
dark). The water was changed at least once per week. The dissolved oxygen concentration was more than 80% of the air
saturation value.
Zebrafish with at least 9 days of acclimatization and mortality < 5% within these days prior to the start of the exposure were used in
the test.
No mortality was observed during this time. No disease treatments were administered throughout holding and testing.


FEEDING DURING TEST (as applicable)
- Food type: Sera Vipan, Sera GmbH, Germany
- Amount: 4% of fish body weight, fish were not fed 24h before test start
- Frequency: 3 times per week

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Total Hardness: 67

Test temperature:

22.9 - 23.6°C (mean 23.2 °C)

pH:

7.73 - 7.90

Dissolved oxygen:

100% Oxygen saturation

Nominal and measured concentrations:

Nominal concentrations: 8.55, 18.8, 41.3, 90.0, 200 µg/L
Arithmetic mean measured concentrations: 2.52, 4.97, 13.1, 27.3, 78.5 µg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 2.9L glass aquaria, covered by glass lids
- Type (delete if not applicable):glass aquaria were covered by glass lids
- Fill volume: 2.5 L
- Aeration: Dilution water tank was aerated
- Type of flow-through (e.g. peristaltic or proportional diluter): Precision syringe pumps were used for the application of stock solution. The stock solution and the dilution water were mixed in a mixing chamber (approx. Vol. 0.7 L) by magnetic stirring before passing the test aquaria (one replicate per test concentration, control and solvent control) where the fish were exposed.
- Renewal rate of test solution (frequency/flow rate): 2.04 - 2.16 L/h
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): one
- No. of vessels per control (replicates): one
- No. of vessels per vehicle control (replicates): one
- Biomass loading rate:0.0644g fish per liter test solution

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light / dark 8h
- Light intensity: 529 to 743 (mean value 648) Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observations were made after 2 ± 0.5 h and 5 ± 1 h after the start of the exposure. On days 1-4 of the test, all vessels with living fish were inspected twice per day.


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2

Range finding study:
- Test concentrations: First Range finding study (semi-static): 2, 0.2, 0.03 mg/L;
Second range finding study (flow-through): 0.2 mg/L
- Results used to determine the conditions for the definitive study: yes

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

50.5 µg/L

95% CI:

>= 27.3 - <= 78.5

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: The LC50-value after 96 hours was calculated by linear regression from the highest test concentration without mortality and the highest test concentration with 86% mortality.

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

27.3 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 78.5 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The test substance showed a very steep concentration response curve.
The highest test concentration of 78.5 µg/L arithmetic mean measured (200µg/L nominal) resulted in 86% mortality. In the next lower concentration of 27.3 µg/L arithmetic mean measured (90.0 µg/L nominal) no mortality was observed.

Reported statistics and error estimates:

The LC50-value after 96 hours was calculated by linear regression from the highest test concentration without mortality and the highest test concentration with 86% mortality.

Validity criteria fulfilled:

yes

Conclusions:

In this study the test substance was found to cause lethal effects to Zebrafish after 96 hours at a nominal test item concentration of 200 µg/L, corresponding to an arithmetic mean measured concentration of 78.5 µg/L. The LC50 after 96 hours was 144 mg test item/L (nominal concentration), corresponding to an arithmetic mean measured concentration of 50.5 mg/L. The LC0 and LC100 after 96 hours were 90.9 and > 200 mg test item/L (nominal test item concentration), respectively, corresponding to arithmetic mean measured concentrations of 27.3 and > 78.5 µg/L. The LC50-value after 96 hours was calculated by linear regression from the highest test concentration without mortality and the highest test concentration with 86% mortality.

Executive summary:

A valid guideline study according to OECD 203 over 96h resulted in a LC50 value of 50.5 µg/L (arithmetic mean measured concentration).

Due to the volatility/instability of the substance the test was done in a flow-throgh design and aquaria were covered with glass lids.

Description of key information

LC50 (96 h): 0.0505 mg/L(OECD 203).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

0.051 mg/L

Additional information

Experimental data on the acute toxicity of the test substance to fish is available. A valid study according to OECD 203 over 96h under flow-through conditions resulted in a 96-hour LC50 value of 0.0505 mg/L. 

Due to the volatility of the test substance the test vessels were covered by glass lids.