L-p-mentha-1(6),8-dien-2-one

Toxicity to terrestrial arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial arthropods, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Data is from peer reveiwed journal

Justification for type of information:

Data is from peer reveiwed journal

Qualifier:

according to guideline

Guideline:

other: refer the principle mentioned below

Principles of method if other than guideline:

Aim of this study was to determine the nature of chemical on the mortality, survival, growth and pupation rate of the larva.

GLP compliance:

not specified

Application method:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Details on preparation and application of test substrate:

- Method: one drop of Triton X-100 was added to 1 ml test chemical and after mixing thoroughly, dilution was effected with water to obtain the desired concentrations. The emulsions were stirred into the diet in a Waring Blendor.

- Chemical name of vehicle (organic solvent, emulsifier or dispersant):
Triton X-100

Test organisms (species):

other: Earias insulana

Details on test organisms:

Weight of organisms: 3 mg

Study type:

laboratory study

Total exposure duration:

6 d

Remarks:

2, 4, 6 days

Details on test conditions:

Details on test conditions
TEST SYSTEM
- Test vessel: screw-cap glass jars
- No. of organisms per vessel: 4-10
- No. of vessels per concentration (replicates): 2-3
- No. of vessels per control (replicates): 2-3
- No. of vessels per vehicle control (replicates): 2-3

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : weighed, and mortality was recorded.


TEST CONCENTRATIONS
- Test concentrations: 500, 1000, 1500, 2500, 5000 and 10000 mg/l

Nominal and measured concentrations:

500, 1000, 1500, 2500, 5000 and 10000 mg/l

Reference substance (positive control):

not specified

Key result

Duration:

6 d

Dose descriptor:

LC100

Effect conc.:

10 000 other: mg/l

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: death observed

Fig: Development of 7-day-old Earias insulana larvae on diets containing different concentrations of test chemical

Concentration of chemical in the diet (mg/l)	No. and average weight of larvae at the beginning of experiment		Mean survival and average weight of the larvae, after days						% Pupation
			2 days		4 days		6 days
	No	Average. wt	% survival	Average. wt	% survival	Average. wt	% survival	Average. wt
500	80	3.4	7.5	4	-	-	-	-	0
1000	80	3.2	20	2.3	-	-	-	-	0
1500	120	2.9	61.7	3	47.6	3.5	23.3	4.1	0
2500	80	3.4	90	4	75	6	63.7	9.5	15
5000	80	3.2	95	3.6	77	5.1	72.8	7.9	30
10000	80	3.2	97.5	6.4	97.5	13.6	93.7	22	52.5
None (control)	160	3.4	94.4	7.4	89.3	14.2	85.6	29.7	76.2

 

-: Indicates complete death (all dead)

Validity criteria fulfilled:

not specified

Conclusions:

Based on the mortality rate of test organisms Earias insulana due to the test chemical the LC100 was 10000 mg/l.

Executive summary:

Aim of this study was to determine the nature of test chemical on the mortality, survival, growth and pupation rate of the larva.

One drop of Triton X-100 was added to 1 ml test chemical and after mixing thoroughly, dilution was effected with water to obtain the desired concentrations. The emulsions were stirred into the diet in a Waring Blendor. Test conducted at 500, 1000, 1500, 2500, 5000 and 10000 mg/l.

Twenty g of the diet was introduced into the bottom of small screw-cap glass jars (base diam and height, 4.5 cm). A 3-cm-diam hole had been cut in the plastic screw-cap and covered with 200-mesh metal wire screen to prevent escape of larvae but provide ventilation. Seven-day-old larvae, each weighing about 3 mg, were introduced into the jars. Larval weight and the pupation rate were the criteria used to evaluate the activity of the different carvone concentrations. For this the test tubes were examined regularly every second day, larvae were removed and weighed, and mortality was recorded. Based on the mortality rate of test organisms Earias insulana due to the test chemical  the LC100 was 10000 mg/l.

Description of key information

Aim of this study was to determine the nature of test chemical on the mortality, survival, growth and pupation rate of the larva.

One drop of Triton X-100 was added to 1 ml test chemical and after mixing thoroughly, dilution was effected with water to obtain the desired concentrations. The emulsions were stirred into the diet in a Waring Blendor. Test conducted at 500, 1000, 1500, 2500, 5000 and 10000 mg/l.

Twenty g of the diet was introduced into the bottom of small screw-cap glass jars (base diam and height, 4.5 cm). A 3-cm-diam hole had been cut in the plastic screw-cap and covered with 200-mesh metal wire screen to prevent escape of larvae but provide ventilation. Seven-day-old larvae, each weighing about 3 mg, were introduced into the jars. Larval weight and the pupation rate were the criteria used to evaluate the activity of the different test chemical concentrations. For this the test tubes were examined regularly every second day, larvae were removed and weighed, and mortality was recorded. Based on the mortality rate of test organisms Earias insulana due to the test chemical  the LC100 was 10000 mg/l.

Additional information

Aim of this study was to determine the nature of test chemical on the mortality, survival, growth and pupation rate of the larva.

One drop of Triton X-100 was added to 1 ml test chemical and after mixing thoroughly, dilution was effected with water to obtain the desired concentrations. The emulsions were stirred into the diet in a Waring Blendor. Test conducted at 500, 1000, 1500, 2500, 5000 and 10000 mg/l.

Twenty g of the diet was introduced into the bottom of small screw-cap glass jars (base diam and height, 4.5 cm). A 3-cm-diam hole had been cut in the plastic screw-cap and covered with 200-mesh metal wire screen to prevent escape of larvae but provide ventilation. Seven-day-old larvae, each weighing about 3 mg, were introduced into the jars. Larval weight and the pupation rate were the criteria used to evaluate the activity of the different test chemical concentrations. For this the test tubes were examined regularly every second day, larvae were removed and weighed, and mortality was recorded. Based on the mortality rate of test organisms Earias insulana due to the test chemical  the LC100 was 10000 mg/l.