Reaction mass of disodium sulphide and sodium carbonate and sodium hydroxide

Long-term toxicity to fish

Administrative data

Endpoint:

fish early-life stage toxicity

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reaction mass of disodium sulphide, sodium carbonate and sodium hydroxyde

IUPAC Name:

Reaction mass of disodium sulphide, sodium carbonate and sodium hydroxyde

Test material form:

gas under pressure: refrigerated liquefied gas

Details on test material:

Solution containing:
70% Sodium sulphide
17% Sodium hydroxyde
17% Sodium carbonate

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

As recommend in OECD 210

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

33 d

Test conditions

Hardness:

Between 83.64 and 85.68 mg/L

Test temperature:

Between 24.6 and 25.3°C

pH:

between 7..5 and 7.7

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

33 d

Dose descriptor:

NOEC

Effect conc.:

ca. 1.3 mg/L

Effect concentrations 2

Duration:

33 d

Dose descriptor:

LOEC

Effect conc.:

ca. 2.5 mg/L

Details on results:

The cumulative mortality of Dania rerio observed for the range-finding and definitive tests, at each day, are shown on tables 3 and 4, respectively.
No sublethal effects on fish were observed in all the tested concentrations.
The surviving larvae showed normal appearance and behavior during the definitive test.
The survival rate of eggs and larvae of the control was 100%, demonstrating the validity of the results (survival should be at least 70 and 75%, respectively) (Table 9).
The humid weight and size of exposed surviving larvae at concentrations ranged from 46.55 to
47.75 mg and 11.0 to 11.7 mm, respectively (Table 12). The weight and size of the larvae of each replicate were recorded and are shown on tables 10 and 11. The test substance in concentrations had no significant effect on weight and size of the larvae.

The no observed effect concentration (NOEC) of SODIUM SULFIDE SOLUTION over the survival and growth of fish larvae was 1.3 mg.L•1 and the lowest observed effect concentration (LOEC) was 2.5 mg.L-1 in 33 days exposure period.

Water chemical parameters measured in the dilution water and in the test solutions are show on tables 13 and 14, respectively.
The 168-hour LCso value for the reference substance potassium dichromate was 145.20 mg.L-1 (confidence limits at 95% = 129.31 to 163.03 mg.L-1 .

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The value NOEC 33 days of product, SODIUM SULFIDE SOLUTION was 1.3 mg/L.
Not be considered as toxic for fish species