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EC number: 931-740-0 | CAS number: -
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
The 14-day acute toxicity study to earthworms (Eisenia fetida) in artificial soil; LC50 323.20 mg/kg soil dry weight (CL: 290.81 – 359.20) and the NOEC related to mortality and related to biomass change; 250 mg/kg soil dry weight (OECD 207 in accordance with GLP).This study was conducted as a range finding study for earthworm reproduction toxicity study (OECD 222).
The key values for CSA were selected based on the lowest endpoint results of OECD 222. In this study the mortality, behavioural effects, weight change after 28 days, and the number of juveniles (reproduction) after 56 days were assessed on earthworm (Eisenia fetida). Test species were adult worms (with clitellum and weight 266-598 mg), less than one year old from in-house culture, and the test concentrations were control, 18.75; 37.5; 75; 150 and 300 mgTest Item/kg soil dry weight prepared in OECD 222 artificial soil.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 270.92 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 150 mg/kg soil dw
Additional information
The acute toxicity study to earthworms (Eisenia fetida) in artificial soil was conducted for the substance according to the OECD 207 in accordance with GLP. The LC50 323.20 mg/kg soil dry weight with condidence limits: 290.81 – 359.20. The NOEC related to mortality and related to biomass change was 250 mg/kg soil dry weight. This study was conducted as a range finding study for earthworm reproduction toxicity study (OECD 222).
The key values for CSA were selected based on the lowest endpoint results of OECD 222. In this study the mortality, behavioural effects, weight change after 28 days, and the number of juveniles (reproduction) after 56 days were assessed on earthworm (Eisenia fetida). Test species were adult worms (with clitellum and weight 266-598 mg), less than one year old from in-house culture, and the test concentrations were control, 18.75; 37.5; 75; 150 and 300 mgTest Item/kg soil dry weight prepared in OECD 222 artificial soil.
Based on the key study results, the 56-d EC50, based on the reproduction rate (juvenile numbers) of Eisenia fetida was determined as 270.92 mg/kg soil dry weight (95 % confidence limits: 203.86 - 360.03 mg/kg soil dry weight). The 28-d LC50 was determined as 287.43 mg/kg soil dry weight(95 % confidence limits: 257.03 – 329.11 mg/kg soil dry weight).
Based on the adult mortality and biomass development, the NOEC (28 day) was 150 mg Test Item/kg soil dry weight and the NOAEC (56 day) was 150 mg Test Item/kg soil dry weight based on reproduction.
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