Reaction mass of 2,2'-[methylenebis(oxymethylene)]bis[2-ethylpropane-1,3-diol]and propylidynetrimethanol and 2,2'-[oxybis(methylene)]bis[2-ethylpropane-1,3-diol]

Endpoint summary

Description of key information

In a 48 hr GLP acute toxicity study, Daphnia magna showed to have an EC₅₀ value greater than 100 mg HB TMP/L.

 

In a 72 hour GLP acute toxicity study, cultures of Desmodesmus subcapitus showed to have a 72 -hour E_yC₅₀ (Cell density/biomass) and E_rC₅₀ (growth rate) greater than 100 mg HB TMP/L.

 

In a 3 hour GLP study performed according to OECD 209 (2010), the effect of HB TMP on the respiration of activated sewage sludge micro-organisms gave a 3 Hour EC₅₀ value of greater than 1000 mg/L.  The No Observed Effect Concentration (NOEC) after 3 hours exposure was 1000 mg/L.

Additional information

The 48 hr-acute toxicity of HB TMP to Daphnia magna flow through conditions.  Test species were exposed to potassium dichromate and the test chemical at nominal concentration of 0, 4, 6, 10, 22, 46, and 100 mg/L for 48 hr.  Immobilisation and sublethal effects were observed daily.  The 48 hour EC₅₀ was greater than 100 mg/l. Based on the results of this study, HB TMP would not be classified as toxic to Daphnia magna to in accordance with the CLP classification.

 

In a 72 hour GLP acute toxicity study, cultures of Desmodesmus subcapitus were exposed to HB TMP at nominal concentrations of 0 (control), 1, 3.2, 10, 32, and 100mg/L flow-through conditions in accordance with the OECD guideline 201 (Version 23 March 2006).  Two experiments were performed. In the 1st experiment, both validity criteria (coefficient of variation of the daily growth rates and coefficient of variation of average growth) were not met. Therefore, the experiment was aborted and repeated under the same conditions like the first experiment. The results of the 1st experiment are not stated, but will be kept together with the other raw data in the GLP archive of the test facility. There were no compound related phytotoxic effects. Based on the nominal test concentrations, both the 72-hour E_yC₅₀(cell density/biomass) and E_rC₅₀(growth rate) were determined to be greater than 100 mg/L.

 

In a 3 hour GLP study performed according to OECD 209 (2010), activated sewage sludge was exposed to an aqueous dispersion of HB TMP at concentrations of 0, 10, 100 and 1000 mg/L for a period of 3 hours. Less than 13% inhibition was observed in all concentrations. The 3-hour EC₅₀ was estimated to be >1000 mg/L, and the 3-hour NOEC was 1000 mg/L.