Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 942-381-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study in compliance with GLP-criteria.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 72008-25-4
- EC Number:
- 615-703-3
- Cas Number:
- 72008-25-4
- IUPAC Name:
- 72008-25-4
- Details on test material:
- - Name of test material (as cited in study report): Asta-C6-Acetal rein
- Physical state: liquid, yellowish
- Homogeneity: homogeneous
- Storage: room temperature, exclusion of light.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples taken just before test solution renewal (at ca. 24h, 48h 72h and 96h) and at teh start of the test (ca. 1 h).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- For the first substance preparation (t=0), to obtain test concentrations of 4.64, 10, 21.5 and 46.4 mg/L, test substance at 0.0465, 0.1001, 0.2152, 0.4641 and 1.00 g were added to the test water using an ultra-turrax stirrer.
For the second substance preparation (t=24h), to obtain test concentrations of 4.64, 10, 21.5 and 46.4 mg/L, test substance at 0.0465, 0.1004, 0.2153 and 0.4644 g were added to the test water using an ultra-turrax stirrer.
For the third substance preparation (t=48h), to obtain test concentrations of 4.64, 10, 21.5 and 46.4 mg/L, test substance at 0.0464, 0.1001, 0.2151 and 0.4640 g were added to the test water using an ultra-turrax stirrer.
For the fourth substance preparation (t=72h), to obtain test concentrations of 4.64, 10, 21.5 and 46.4 mg/L, test substance at 0.0462, 0.1006, 0.2151 and 0.4642 g were added to the test water using an ultra-turrax stirrer.
From the beginning of preparing the test solutions until termination of placing the fish into the aquaria 20 minutes were needed.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- GENERAL INFORMATION:
- Animal Species: Zebra fish (Brachydanio rerio RAM. and BUCH.)
- Consignment dated: Jun. 24, 1997
- Animal. breeder/suppijer: Charles River Aquatics, Someren; Netherlands
- Hatching date: April 03, 1997
- Age of the animals: About 3 months
- Body length: 3.67 (3.2 - 3.9) cm
- Body weight: 0.55 (0.3 - 0.8) g
HOUSING:
- The fish were kept in a flow-through tank in tap water, not chlorinated, passed through a charcoil. filter and aerated with oil-free air.
-Duration of housing: About 2 months
- Photoperiod: 16 hours light and 8 hours dark
- Total hardness: About 2.5 mmol/L (= about 250 mg/L CaCO3)
- Acid capacity: About 5.5 mmol/L
- Oxygen content: > 60% of maximum saturation
- pH: About 8.0
- Water temperature: About 22 - 23 °C
- Mortality during the last 14 days: 0 %
- Medical treatment: none
- Diet: 'TETRA MIN' standard feed for aquarium fish (TETRA - WERKE, MELLE, FRG) ad libitum; and on workdays generally additionally live artemia.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 2.5 mmol/L (about 250 mg/L CaCO3)
- Test temperature:
- 22 - 23 °C
- pH:
- 8.1-8.5
- Dissolved oxygen:
- 5.4-8.5 mg/L
- Nominal and measured concentrations:
- Nominal concentrations:
0 (control), 4.64, 10, 21.5, 46.4 and 100 mg/L.
Measured concentrations (just before test medium renewal):
- After 24h: n.d., 3.1, 6.5, 16.1, 32.8 and 75.2 mg/L.
- After 48 h: n.d., 2.4, 6.5, 14.9 and 35.6 mg/L (nominal conc. of 100 mg/L not analysed).
- After 96h: n.d., 3.0, 7.2, 15.4 and 36.8 mg/L (nominal conc. of 100 mg/L not analysed). - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10-L all-glass aquaria, open, 30 x 22 x 24 cm
- Aeration: aeration with oil-free air
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.6 g fish /L
TEST MEDIUM / WATER PARAMETERS
Municipal water of the City of Frankenthal, not chlorinated and passed through a charcoal filter, aerated
OTHER TEST CONDITIONS
- Photoperiod: 16:8 hour light:dark
- Adaptation: Test conditions corresponded to housing conditions.
- Withdrawal of food: 1 day before and during exposure.
EXAMINATIONS:
- Mortality: at 1, 24, 48, 72, and 96 hours
- Symptoms: apathy, abdominal distension, exophtalmos, escape reflex, hyperreflexia, convulsions, gasping, narcotic-like state, tumbling, restlessness, discoloration, headstand and accelerated respiration at 1, 24, 48, 72, and 96 hours
- the analytical concentrations after 1, 24, 48, 96 and 1+24+48+96 hours. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 21 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.L.: 14.3-32.6 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 27.35
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% C.L.: 19.46-38.22 mg/L
- Details on results:
- Mortality occurred first at the nominal concentration 21.5 mg/L.
Other substance-related effects were: apathy, convulsians, narcotic-like state, tumbling, swimming near water surface, swimming near the bottom. - Reported statistics and error estimates:
- Finney, D.J., Probit Analysis, Cambr. Univ. Press, 3rd ed., 1971; certain aspects of this method have been modified. The exact procedure can be
seen from the Standard Operating Procedures (SOPs) of the laboratory.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Mortality:
Nominal conc. (mg/L)
Number of fish
Mortality (cumulated) after
1h
4h
24h
48h
72h
96h
0
10
0
0
0
0
0
0
4.64
10
0
0
0
0
0
0
10
10
0
0
0
0
0
0
21.5
10
0
0
0
0
1
4
46.4
10
0
0
2
3
7
8
100
10
7
10
10
10
10
10
Analytically determined concentrations:
Nominal conc. (mg/L)
Measured conc. (mg/L) and recovery (%) after:
Aritmetic mean measured conc. (mg/L)
1h
24h
48h
96h
1h+24h+48h+96h
0
n.d.
n.d.
n.d.
n.d.
-
4.64
3.6
(77.6%)
3.1
(66.8%)
2.4
(51.7%)
3.0
(64.7%)
3.0
10
8.6
(86.0%)
6.5
(65.0%)
6.5
(65.0%)
7.2
(72.0%)
7.2
21.5
18.9
(87.9%)
16.1
(74.9%)
14.9
(69.3%)
15.4
71.6%)
16.3
46.4
41.0
(88.4%)
32.8
(70.7%)
35.6
(76.7%)
36.8
(79.3%)
36.5
100
91.4
(91.4%)
75.2
(75.2%)
n.a.
-
n.a.
-
83.3
n.d. = not detectable
n.a. = not analysed
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.