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EC number: 932-389-6 | CAS number: -
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980-09-25 to 1981-08-03
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study similar go guideline and under GLP with minor restrictions. Only tested to 2500 µg/plate and no cross-linking sensitive tester strain used (e.g. TA102 or E.coli WP2)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- no cross-linking sensitive tester strain used (e.g. TA102 or E.coli WP2)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Reaction mass of (R)-cyano(3-phenoxyphenyl)methyl rel-(1R,3R)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate and (R)-cyano(3-phenoxyphenyl)methyl rel-(1S,3S)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate
- EC Number:
- 932-389-6
- Molecular formula:
- C23H19ClF3NO3
- IUPAC Name:
- Reaction mass of (R)-cyano(3-phenoxyphenyl)methyl rel-(1R,3R)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate and (R)-cyano(3-phenoxyphenyl)methyl rel-(1S,3S)-3-((1Z)-2-chloro-3,3,3-trifluoroprop-1-en-1-yl)-2,2-dimethylcyclopropanecarboxylate
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with
- Metabolic activation system:
- S9 mix from Aroclor1254 induced rat liver
- Test concentrations with justification for top dose:
- 4, 20, 100, 500, 2500 µg/plate
- Vehicle / solvent:
- - Vehicle/solvent used: DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene, N-Methyl-N'-nitro-N-nitrosoguanidine, 9-Aminoacridine, 4-Nitroquinoline N-oxide
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 3-4 d
NUMBER OF REPLICATIONS: 3 plates per dose - Evaluation criteria:
- A positive response was taken to be a two-fold or greater increase in the mean number of revertant colonies appearing over background reversion rate together with evidence of a dose response.
- Statistics:
- Mean and standard deviation of three plates per dose group were determined.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: observed at 2500 µg/plate in test No. 2
Any other information on results incl. tables
Table: Results of the Ames test with Cyhalothrin
Strain |
S9 |
Concentration [µg/plate] |
Test 1 |
|
Test 2 |
|
Mean ± SD |
Ratio |
Mean ± SD |
Ratio |
|||
TA1535 |
+ |
Solvent control |
25.2±5.7 |
|
31.4±6.1 |
|
2500 |
14.7±4.6 |
0.6 |
24.7±4.6 |
0.8 |
||
500 |
14.3±4.7 |
0.6 |
23.7±5.5 |
0.8 |
||
100 |
15.3±2.9 |
0.6 |
24.3±4.9 |
0.8 |
||
20 |
19.3±3.2 |
0.8 |
28.7±3.2 |
0.9 |
||
4 |
18.0±6.7 |
0.7 |
31.3±0.6 |
1.0 |
||
TA1535 |
- |
Solvent control |
43.6±3.5 |
|
17.6±6.3 |
|
2500 |
42.3±9.7 |
1.0 |
127.3±129.5 |
7.2 |
||
500 |
40.7±7.8 |
0.9 |
16.7±7.4 |
0.9 |
||
100 |
48.3±12.1 |
1.1 |
22.0±5.2 |
1.3 |
||
20 |
51.0±12.0 |
1.2 |
23.7±8.5 |
1.3 |
||
4 |
49.3±17.4 |
1.1 |
18.7±4.2 |
1.1 |
||
TA1537 |
+ |
Solvent control |
35.8±5.8 |
|
23.4±4.0 |
|
2500 |
19.3±2.5 |
0.5 |
22.7±1.2 |
1.0 |
||
500 |
21.3±3.1 |
0.6 |
20.5±8.7 |
0.9 |
||
100 |
29.0±6.1 |
0.8 |
22.3±4.0 |
1.0 |
||
20 |
24.0±3.6 |
0.7 |
21.0±7.5 |
0.9 |
||
4 |
28.0±3.6 |
0.8 |
21.0±3.0 |
0.9 |
||
TA1537 |
- |
Solvent control |
14.8±3.9 |
|
13.0±2.2 |
|
2500 |
10.3±4.5 |
0.7 |
10.7±2.9 |
0.8 |
||
500 |
16.0±5.0 |
1.1 |
13.0±3.0 |
1.0 |
||
100 |
19.3±4.5 |
1.3 |
16.0±4.0 |
1.2 |
||
20 |
12.3±1.2 |
0.8 |
12.3±4.0 |
0.9 |
||
4 |
11.7±4.0 |
0.8 |
14.3±2.9 |
1.1 |
||
TA1538 |
+ |
Solvent control |
58.8±14.1 |
|
47.4±5.1 |
|
2500 |
35.3±2.1 |
0.6 |
26.3±10.5 |
0.6 |
||
500 |
48.7±9.0 |
0.8 |
39.0±5.7 |
0.8 |
||
100 |
56.7±0.6 |
1.0 |
62.0±4.0 |
1.3 |
||
20 |
63.0±9.6 |
1.1 |
61.3±5.5 |
1.3 |
||
4 |
52.3±17.6 |
0.9 |
51.0±14.9 |
1.1 |
||
TA1538 |
- |
Solvent control |
32.8±1.3 |
|
15.6±5.0 |
|
2500 |
19.0±4.6 |
0.6 |
14.0±6.1 |
0.9 |
||
500 |
25.7±14.6 |
0.8 |
13.7±1.2 |
0.9 |
||
100 |
16.0±7.2 |
0.5 |
27.0±18.4 |
1.7 |
||
20 |
19.0±5.6 |
0.6 |
14.3±7.8 |
0.9 |
||
4 |
27.7±1.2 |
0.8 |
22.3±5.0 |
1.4 |
||
TA98 |
+ |
Solvent control |
50.0±6.7 |
|
47.0±7.3 |
|
2500 |
38.3±5.0 |
0.8 |
51.5±6.4 |
1.1 |
||
500 |
44.0±3.0 |
0.9 |
49.3±12.2 |
1.0 |
||
100 |
42.0±1.7 |
0.8 |
48.3±1.2 |
1.0 |
||
20 |
46.3±10.8 |
0.9 |
54.0±2.6 |
1.1 |
||
4 |
43.7±7.6 |
0.9 |
50.7±9.5 |
1.1 |
||
TA98 |
- |
Solvent control |
31.8±4.7 |
|
23.8±5.1 |
|
2500 |
20.8±6.9 |
0.6 |
29.3±9.0 |
1.2 |
||
500 |
22.7±4.5 |
0.7 |
31.0±10.8 |
1.3 |
||
100 |
26.3±6.8 |
0.8 |
29.3±2.1 |
1.2 |
||
20 |
19.0±4.4 |
0.6 |
29.0±1.0 |
1.2 |
||
4 |
24.0±1.7 |
0.8 |
22.7±4.0 |
1.0 |
||
TA100 |
+ |
Solvent control |
140.8±9.9 |
|
195.8±16.4 |
|
2500 |
120.0±15.5 |
0.9 |
189.7±2.5 |
1.0 |
||
500 |
125.3±23.9 |
0.9 |
176.3±24.0 |
0.9 |
||
100 |
116.3±15.3 |
0.8 |
182.3±16.6 |
0.9 |
||
20 |
135.3±11.0 |
1.0 |
188.5±20.5 |
0.9 |
||
4 |
113.3±10.7 |
0.8 |
171.7±20.4 |
0.9 |
||
TA100 |
- |
Solvent control |
116.8±15.4 |
|
96.8±12.5 |
|
2500 |
74.7±6.4 |
0.6 |
107.3±15.0 |
1.1 |
||
500 |
104.3±14.6 |
0.9 |
120.0±19.7 |
1.2 |
||
100 |
85.0±10.4 |
0.7 |
107.7±11.6 |
1.1 |
||
20 |
97.0±11.0 |
0.8 |
105.0±18.5 |
1.1 |
||
4 |
79.0±14.7 |
0.7 |
98.3±16.4 |
1.0 |
Applicant's summary and conclusion
- Conclusions:
- The test item Cyhalothrin was found to be not mutagenic in the bacterial reverse mutation assay (Ames test) with the tester strains TA1535, TA1537, TA1538, TA98, and TA100 with or without metabolic activation using S9 mix from Aroclor1254 induced rat liver.
- Executive summary:
The mutagenic potential of Cyhalothrin was assessed in a bacterial reverse mutation assay (Ames test). The test was performed similar to OECD Guideline 471 using Salmonella typhimurium strains TA1535, TA1537, TA1538, TA98, and TA100. Deviation from the actual guideline was the lack of cross-linking tester strains such as TA102 or E. coli WP2 and only tested to 2500 µg/plate. GLP compliance was stated.
The test item Cyhalothrin was found to be not mutagenic in the bacterial reverse mutation assay (Ames test) with the tester strains TA1535, TA1537, TA1538, TA98, and TA100 with or without metabolic activation using S9 mix from Aroclor1254 induced rat liver.
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