Naphthenic acids, reaction products with diethylenetriamine

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2010

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil

Test material

Test material information

Constituent 1

Reference substance name:

Naphthenic acids, reaction products with diethylenetriamine

EC Number:

268-610-1

EC Name:

Naphthenic acids, reaction products with diethylenetriamine

Cas Number:

68131-13-5

Molecular formula:

C16H21N3O2 (minimal)

IUPAC Name:

Naphthenic acids, reaction products with diethylenetriamine

Details on test material:

Name: MK195KSF-Naphthenic acids, reaction products with diethylenetriamine
The test substance was received at Brixham Environmental Laboratory on 1 June 2010 and
assigned the Brixham test substance number 10-0152. The test substance (Batch Number
MK195K/BM/198/067/A/001) was supplied as a hazy solid. A certificate of analysis (dated
19 July 2010, Appendix 2) stated the sample had a purity/concentration of 100%.
The sample was stored in the dark, at ambient temperature, in the container in which it was
received until required for testing, when an appropriate subsample was provided for the test
operator.

Radiolabelling:

no

Study design

Test temperature:

between 20.62 and 22.70°C

HPLC method

Details on study design: HPLC method:

An HPLC system comprising the following components was set up;
HPLC pump
Injection valve fitted with 100 μL loop
25 cm × 4.6 mm id column packed with a 5 μm CN phase
Photo diode array detector extracted at 198 and 220 nm
Chromatography data system
Mobile phase
The mobile phase consisted of 55% methanol and 45% pH 2 or 10 adjusted water (depending
on the required pH). The mobile phase was pumped through at a flow rate of 1.5 mL min-1.

Batch equilibrium or other method

Analytical monitoring:

no

Results and discussion

Adsorption coefficient

Type:

log Koc

Value:

< 1.5

Remarks on result:

other: at pH 2 and pH 10. Temperature range 20.62 to 22.70°C.

Results: HPLC method

Details on results (HPLC method):

The test described has been validated for the quantitative estimation of log10KOC values in the
range 1.5 to 5.0. In those instances where the statistical treatment of the experimental
data produces a calculated log10KOC value outside of this range then the final result is quoted
as either <1.5 or >5.0 as appropriate.
At pH 2, the k′ for MK195KSF was calculated for 10 mg L-1 test concentration only. The k′
value for a 10 mg L-1 standard was -0.449. Therefore, the estimated log10 KOC for MK195KSF
at pH 2 is < 1.5.
At pH 10, the k′ for MK195KSF was calculated for both 5.0 and 10 mg L-1 test
concentrations. The k′ values for 5.0 and 10 mg L-1 standards were 0.367 and 0.366,
respectively. Therefore, the estimated log10 KOC for MK195KSF at pH 10 is < 1.5.
The pH of the mobile phase was nominally 2 (pH 2 adjusted water measured at 2.55) and 10
(pH 10 adjusted water measured at 9.98). The temperature of the room in which the HPLC
work was done ranged between 20.62 to 22.70 °C.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test described is validated for the quantitative estimation of
log10KOC values in the range 1.5 to 5.0. In those instances where the
statistical treatment of the experimental data produces a log10KOC value
outside of this range then the final result is quoted as either <1.5 or
>5.0.