4'-aminoazobenzene-4-sulphonic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 62.5 mg, 125 mg, 250 mg ,500mg & 100 mg of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.5 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish
- Strain:Danio rerio
- Source:Orange City Aquarium, Sitabuldi, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD): average 2.28 cm
- Weight at study initiation (mean and range, SD): average 0.116 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 12 hours light and 12 hours darkness
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

42.5 mg of CaCO3

Test temperature:

20-33 °C

pH:

7

Dissolved oxygen:

5.7 - 7.4 mg/l

Nominal and measured concentrations:

6.25 mg/L, 12.5mg/L, 25 mg/L, 50 mg/L and 100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No effect were observed

Sublethal observations / clinical signs:

Table 1: Mortality and symptoms:

Nominal conc. (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	-	-	-	-
6.5	8	-	-	-	-
12.5	8	-	-	-	-
25	8	-	-	-	-
50	8	-	-	-	-
100	8	8	-	-	-

Visible Symptoms: No visible symptoms were observed

Nominal conc. (mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS8	NS8	NS8
6.5	8	NS8	NS8	NS8	NS8
12.5	8	NS8	NS8	NS8	NS8
25	8	NS8	NS8	NS8	NS8
50	8	NS8	NS8	NS8	NS8

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

Table 2: pH and oxygen content:

Nominal conc. (mg/l)	pH readings after
	24h	48h	72h	96h
0 (control)	7.2	7.5	7.8	7.9
6.5	7.8	7.7	7.6	7.9
12.5	7.8	7.5	7.6	7.7
25	7.8	7.6	7.8	8.1
50	6.9	7.4	7.5	7.5

Nominal conc. (mg/l)	Oxygen content (mg/l) after
	24h	48h	72h	96h
0 (control)	6.77	6.61	6.88	7.04
6.5	5.89	6.61	6.34	6.81
12.5	5.81	6.54	6.25	6.90
25	5.82	6.48	6.1	6.65
50	5.7	6.61	6.08	6.47

Table 3: Test temperature:

Nominal conc. (mg/l)	Temperature (°C) after
	24h	48h	72h	96h
0 (control)	24	27.6	24.8	21.1
6.5	29.7	26.9	27.8	22.7
12.5	30.0	26.9	28.0	23.0
25	29.7	27.0	28.3	23.3
50	29.9	27.0	28.4	23.5

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-0 (96 h)] for test chemical on Zebra Fish Danio rerio was determine to be 50 mg/l based on the mortality of fish and after 24 hrs, LC100 was observed at 100 mg/l.

Executive summary:

Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 62.5 mg, 125 mg, 250 mg ,500mg & 100 mg of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.5 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Plastic aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Aeration in test vessels was provided 1 day before the start of experiment. After 96 hours of exposure of test item to various nominal test concentrations, LC0 was determine to be 50 mg/l on the basis of mortality effect observation and after 24 hrs, LC100 was observed at 100 mg/l.

Description of key information

Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 62.5 mg, 125 mg, 250 mg ,500mg & 100 mg of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.5 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Plastic aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Aeration in test vessels was provided 1 day before the start of experiment. After 96 hours of exposure of test item to various nominal test concentrations, LC0 was determine to be 50 mg/l on the basis of mortality effect observation.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

50 mg/L

Additional information

Based on the short term toxicity studies available for the test chemical, studies were reviewed to determine the toxic nature of test chemical on the mortality of fish. The studies are as mentioned below:

 

Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 62.5 mg, 125 mg, 250 mg ,500mg & 100 mg of the test substance in 10 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.5 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L, respectively. Potable water (passed through reverse osmosis system) was used and Zebra Fish Danio rerio were exposed to these concentration for 96 hours. Plastic aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. Aeration in test vessels was provided 1 day before the start of experiment. After 96 hours of exposure of test item to various nominal test concentrations, LC0 was determine to be 50 mg/l on the basis of mortality effect observation.