3,5-DICARBOXYPHENYLBORONIC ACID

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

Minor deviation with no impact on study reliability

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

5-(dihydroxyboranyl)benzene-1,3-dicarboxylic acid

Cas Number:

881302-73-4

Molecular formula:

C8H7BO6

IUPAC Name:

5-(dihydroxyboranyl)benzene-1,3-dicarboxylic acid

Test material form:

solid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

20°C ± 2°C

pH:

between 6.3 and 7.8

Dissolved oxygen:

between 8.0 and 8.4 mg/L

Nominal and measured concentrations:

Since the deviation between the measured concentrations and the nominal concentrations was
within the range of ± 20 %, analysis of the results were based in the nominal concentrations.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

>= 73.6 - <= 86.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC10

Effect conc.:

>= 73.6 - <= 86.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 with reference item over a period of 24 hours (No GLP results):
*Actual value: 1.65 mg/L
*Reference value: between 0.6 and 2.1 mg/L
*Compliance: yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute effects of 3,5-DICARBOXYPHENYLBORONIC ACID at the definitive nominal concentrations of 0; 11.96; 20.36; 34.6; 58.84 and 100.0 mg test item.L-1 to Daphnia magna were investigated under laboratory conditions. Validity criteria are met. Results show that EC50-48H is greater than 100 mg/L for the test item.

Executive summary:

The aim of this study was to assess the effects of the test item 3,5-DICARBOXYPHENYLBORONIC ACID on the mobility of Daphnia magna over a period of 48 hours under static conditions according to OECD Guideline 202 (April 2004). Daphnia magna is a freshwater crustacean that is recommended by the OECD Guideline 202.
The test item is a soluble product under the test conditions and the test solutions were prepared in the test medium by dilution of a primary stock solution of test item.
The acute effects of 3,5-DICARBOXYPHENYLBORONIC ACID at the definitive nominal concentrations of 0; 11.96; 20.36; 34.6; 58.84 and 100.0 mg test item.L-1 to Daphnia magna were investigated under laboratory conditions.
The analytical samples were sampled at the beginning of the test and at the end of the test in the content of the vessels pooled and homogenised.
Since the deviation between the measured concentrations and the nominal concentrations was within the range of ± 20 %, analysis of the results was based in the nominal concentrations.

EC50-24h (95% confidence limits)	>100 mg/L
EC50-48h (95% confidence limits)	>100 mg/L
EC10-24h (95% confidence limits)	80.0 mg/L (73.6 – 86.5)
EC10-48h (95% confidence limits)	80.0 mg/L (73.6 – 86.5)