7,14,25,32-tetraazaundecacyclo[21.13.2.2²,⁵.0³,¹⁹.0⁴,¹⁶.0⁶,¹⁴.0⁸,¹³.0²⁰,³⁷.0²⁴,³².0²⁶,³¹.0³⁴,³⁸]tetraconta-1(36),2(40),3,5(39),6,8(13),9,11,16,18,20,22,24,26(31),27,29,34,37-octadecaene-15,33-dione; 7,14,25,32-tetraazaundecacyclo[21.13.2.2²,⁵.0³,¹⁹.0⁴,¹⁶.0⁶,¹⁴.0⁸,¹³.0²⁰,³⁷.0²⁵,³³.0²⁶,³¹.0³⁴,³⁸]tetraconta-1(36),2(40),3,5(39),6,8(13),9,11,16,18,20,22,26(31),27,29,32,34,37-octadecaene-15,24-dione

Bioaccumulation: aquatic / sediment

Description of key information

Accumulation in organisms is not to be expected.

Additional information

The bioaccumulation potential of Perylene Black I (EC 479 -300 -2) was assessed in a weight of evidence approach using logPow, Critical body burden and a measured BCF.

The represented log Pow values were estimated based on the individual solubilities in n-octanol and in water. The values were summarised from two studies with 2 different methods.

The log Pow value of <2 corresponds to the individual solubilities by the flask method according to OECD Guideline 105 and the log Pow value of >1.05 corresponds to the individual solubilities by the ETAD method, which was developed by intensive cooperation with the ETAD (Ecological and Toxicological Association of Dyes and Organic Pigments Manufacturers) and is regarded as acceptable for pigments.

Estimation of log Pow according to OECD Guideline 105:

Pow= C(n-octanol) / C(water)= 56 µg/L / <5 µg/L = 11.2, thus Log Pow= >1.05

Estimation of log Pow according to ETAD method:

Pow= C(n-octanol) / C(water)= <10 mg/L / <0.1 mg/L = 100, thus Log Pow= <2. Therefore, log Pow is estimated to be >1.05 to <2 at 25°C and bioaccumulation in organisms is not be expected.

To assess the bioaccumulation potential of a substance with low solubility in octanol and water the critical body burden (CBB) can be calculated based on the solubility in octanol. According to “Guidance on Information Requirements and Chemical Safety Assessment Chapter R.11: PBT/vPvB assessment” v 3.0 in R.11.4.2.1.2 octanol is a reasonable surrogate for fish lipid, thus a low substance concentration in octanol may indicate reduced bioconcentration / bioaccumulation potential. It is proposed that where a substance shows no specific mode of action and has a

C_octanol[mg/L] < 0.002 [mMol/L] x Mol weight (g/Mol)

it can be assumed that the compound has only a limited potential to establish high body burdens and to bioaccumulate.

For Perylene Black I (EC 479 -300 -2) the CBB is estimated to be 1.072, based on Molecular mass of 536 g/Mol and C_octanol of 0.056 mg/L. Thus, the substance shows a C_octanol < CBB which indicates that the substance has only a limited potential to establish high body burdens and to bioaccumulate.

For Perylene Black I (EC 479-300-2) a guideline study according to OECD guideline 305 is available. The bioconcentration test was conducted with the test substance in Danio rerio over an uptake period of 28 days followed by a depuration period of 16 days. The fish were exposed in a flow-through system to the test concentrations of 0.1 and 1.0 µg/L, which is below the estimated water solubility of <5µg/L. The stock solution of the test substance was prepared with a solubilizer in order to increase the water solubility of the test substance in the test media, resulting in a unrealistic bioavailability of the substance and as a consequence in a limited reliable BCF-value. However, the used test concentrations are within the water solubility and the result of the study, BCF of 364, can be used in a weight of evidence approach combined with data on logPow and critical body burden.

In conclusion the substance has a low potential for bioaccumulation, thus accumulation in organisms is not to be expected.