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Diss Factsheets
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EC number: 905-459-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-guideline study but conducted under sound scientific principles
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- A radiorespirometric method was used to determine the compatibility of the test substance with secondary waste treatment microorganisms . The
procedure used microorganisms from a laboratory-maintained activated sludge unit. These microorganisms were exposed to [14C] glucose and
the test substance. The extent of conversion of the [14C] glucose to [14C] carbon dioxide in the presence of the test article was compared to
glucose conversion without test article. - GLP compliance:
- not specified
- Remarks:
- pre-GLP
- Analytical monitoring:
- yes
- Details on sampling:
- The intial concentrations of the stock solutions of the test substance was determined. Radioactivity was measured after the 5 hour exposure by liquid scintillation spectrometer.
- Vehicle:
- no
- Details on test solutions:
- Preparation of Stock Test Substance Solutions:
The stock test solutions were prepared in distilled water as follows: 100 mg of the test article was mixed with 1000 mL of distilled water by mechanical stirring for 24 hours at 22°C. The mixture was centrifuged at 10,000xG for 10 minutes . After centrifugation, the top several mLs of liquid was discarded and the remainder saved for use as the stock test article solution. The pH was not measured and no pH adjustment was made. This stock solution subsequently was diluted by a factor of four in the test vessels to achieve the final nominal test article concentrations of (1/4)C, were C
denotes the concentration of the test substance in the stock solution . - Test organisms (species):
- activated sludge
- Details on inoculum:
- Microorganisms were cultured in sludge tanks containing two chambers: a 300 mL aeration/mixing chamber and a 75 mL clarifying chamber. The
contents of the sludge tanks were mixed and aerated by water-saturated air which entered at the bottom of the aeration/mixing chamber. The
microorganisms were maintained on a glucose-peptone- nutrient mixture. This solution vas pumped dropvise into the sludge tank aeration/mixing chamber. The sludge unit had a retention time of 6 hours . Cellular debris, waste products, and excess fluid were drained off at the overflow sidearm located on the settling chamber. Two sludge units were used. Air and nutrients were introduced simultaneously into both sludge tanks . The sludge units were seeded periodically with microorganisms obtained from an industrial waste treatment plant. Seeding occurred once per week, with about 10% of each unit being replaced with new mixed liquor suspended solids (MLSS).
Selection of Microorganisms for Testing:
Between 350 and 400 mL of mixed liquor from the laboratorymaintained domestic activated sludge units were centrifuged in tared 50 mL plastic centrifuge tubes at 7000xG for 5- 15 minutes at 4°C. The supernatant liquid was decanted and the precipitate was washed in 300 mL of oxygenated 0.02M potassium phosphate buffer, pH 6.9. The tube contents were then centrifuged as before, the supernatant liquid was decanted, and the tube and contents were weighed. The contents of the tube were resuspended in sufficient oxygenated potassium phosphate buffer to give a final activated sludge concentration of 10-20 mg/mL . - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 5 h
- Test temperature:
- 27C
- Duration:
- 5 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 0.22 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- other: inhibition of conversion of radiolabeled glucose
- Validity criteria fulfilled:
- yes
- Conclusions:
- Exposure to 0. 22 mg/L of 1,3-Diisopropylbenzene, the highest concentration tested, had no observed inhibitory effects on the microorganisms, that is above the water solubility limit.
- Executive summary:
A radiorespirometric method was used to determine the compatibility of 1,3-Diisopropylbenzene with secondary waste treatment microorganisms. The procedure used microorganisms from a laboratory-maintained activated sludge unit. These microorganisms were exposed to (14C) glucose and 1,3-Diisopropylbenzene. The extent of conversion of the (14C) glucose to (14C) carbon dioxide in the presence of the test article was compared to glucose conversion without test article. Exposure to 0. 22 mg/L of 1,3-Diisopropylbenzene, the highest concentration tested, had no observed inhibitory effects on the microorganisms, that is above the water solubility limit.
Reference
Description of key information
A radiorespirometric method was used to determine the compatibility of 1,3-Diisopropylbenzene with secondary waste treatment microorganisms. The procedure used microorganisms from a laboratory-maintained activated sludge unit. These microorganisms were exposed to (14C) glucose and 1,3-Diisopropylbenzene. The extent of conversion of the (14C) glucose to (14C) carbon dioxide in the presence of the test article was compared to glucose conversion without test article. Exposure to 0. 22 mg/L of 1,3-Diisopropylbenzene, the highest concentration tested, had no observed inhibitory effects on the microorganisms, that is above the water solubility limit.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.