Cyclohexadecen-1-one

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.13 (Bioconcentration: Flow-through fish test)

GLP compliance:

yes (incl. QA statement)

Radiolabelling:

no

Details on sampling:

- Sampling intervals/frequency for test organisms:
- Uptake phase: after 5, 24, 48, 72, 96, 168 h
- Depuration phase: 192, 216, 240, 264 h
- Sampling intervals/frequency for test medium samples:
- Uptake phase: after 5, 24, 48, 72, 96, 168 h
- Depuration phase: 192, 216, 240, 264 h

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: To produce the stock solution (0.2 mg/L), the test substance was weighed into water and treated for 5 minutes at 8000 rpm. with an ultra-turrax. The stock solution and synthetic fresh water were pumped into a mixing chamber. From this mixing chamber, the test solution was directly introduced into the test vessel (nominal test concentration 7 µg/L).
- Controls: synthetic fresh water in accordance with ISO 7346

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Bio International B.V, The Netherlands
- Date of birth: 17 December 1998
- Length at study initiation (range): 2.5 - 3.5 cm
- Weight at study initiation (mean): control: 0.36 g; test concentration: 0.35 g
- Weight at termination (mean): control: 0.30 g; test concentration: 0.34 g
- Lipid content at test initiation: 4.2% of wet weight
- Health status: Mortality while kept < 5 % ; no medical treatment
- Feeding during test
- Food type: T etra Min fish food
- Amount: 2 % of body weight per day
- Frequency: applicated in 2 portions daily

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

174 h

Total depuration duration:

89 h

Hardness:

12.1 °dH

Test temperature:

Acclimation phase: 22.1 - 23.0 °C
Uptake phase: 21.9 - 24.2 °C
Depuration phase: 21.5 - 23.7 °C

pH:

Acclimation phase: 7.9 - 8.1
Uptake phase: 7.6 - 7.8
Depuration phase: 7.8 - 8.1

Dissolved oxygen:

Acclimation phase: 7.4 - 7.7 mg/L
Uptake phase: 7.2 - 8.2 mg/L
Depuration phase: 8.0 - 9.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium
- Type: open
- Size, fill volume: 600 x 295 x 290 mm, 40 L
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): f requency of water renewal: about 10 times per day
- No. of organisms per vessel: 100
- No. of vessels per concentration (replicates): 1
- No. of vessels per control / vehicle control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic fresh water in accordance with ISO 7346

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light / 8 hours dark

Nominal and measured concentrations:

Nomial concentrations: control, 7 µg/L

Reference substance (positive control):

no

Lipid content:

4.2 other: % w.w

Time point:

other: start of the uptake phase

Lipid content:

>= 6.1 - <= 6.9 other: % w.w.

Time point:

other: end of the depuration phase

Key result

Conc. / dose:

7 µg/L

Type:

BCF

Value:

244 L/kg

Basis:

whole body w.w.

Calculation basis:

steady state

Key result

Rate constant:

overall depuration rate constant (d-1)

Value:

-0.035

Details on kinetic parameters:

- Uptake rate constant k(s): The rapid achievement of steady-state conditions during the uptake phase did not allow for a determination of the uptake rate constant.
- Depuration rate constant k(e): After turning to clean water conditions, the measured concentrations in fish fell down to the analytical detection limit within three days approximately.

Details on results:

- Mortality of test organisms: none
- Behavioural abnormalities: none
- Mortality and/or behavioural abnormalities of control: none

Validity criteria fulfilled:

not specified

Conclusions:

The determined BCF of 244 L/kg indicates a low potential of the test substance to bioaccumulate.

Reference 2

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Justification for type of information:

See read-across justification attached to section 13.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

EU Method C.13 (Bioconcentration: Flow-through fish test)

Key result

Conc. / dose:

7 µg/L

Type:

BCF

Value:

244 L/kg

Basis:

whole body w.w.

Calculation basis:

steady state

Description of key information

BCF = 244 L/kg for Danio rerio (EU method C. 13)

Key value for chemical safety assessment

BCF (aquatic species):

244 L/kg ww

Additional information

For the test substance no bioaccumulation study is available. However, the bioaccumulation potential of a suitable surrogate substance in zebra fish Danio rerio was investigated using a flow through system set up according to the EU method C. 13. The fish were exposed via water to the test substance for 174 hours. After a depuration period of 89 hours the test was terminated and a mean growth and lipid corrected BCF of 244 L/kg ww based on the whole body wet weight was determined.