Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study without restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

EC Number:

605-659-3

Cas Number:

173046-61-2

Molecular formula:

Mixture of C6H11O3-[C3H6O]p-[C2H4O]q-[C3H3O]r-C7H14ON and C6H11O3-[C3H6O]p-[C2H4O]q-[C3H3O]s With: 0

IUPAC Name:

Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Appropriate amounts of the test item were weighed separately in glass flasks partially filled with the
Elendt M7 medium and filled up with appropriate volume of the Elendt M7 medium (Table 3)
[SOP/W/7]. The mixtures were visually heterogeneous. Therefore, the mixtures and the control (the
Elendt M7 medium, 700 mL) were mechanically shaken for 24 h (room temperature, 60 revolutions per
minute). After shaking, the mixtures (visually heterogeneous) and the control were left for a settling
phase (room temperature, 2 h). Thereafter, the mixtures (visually heterogeneous) and the control were
filtrated through a conditioned nitrocellulose membrane filter (HAWG filter, 0.45 [m, Millipore),
[SOP/W/37]. This is a WAF-approach followed by filtering which results in a water soluble fraction [9].
Filtration was chosen as the separation method since centrifugation is not possible with the large
volumes of test solution. Each filtrate was visually homogeneous and transparent.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Standard laboratory culture maintained in the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology [SOP/W/67]
- Age at study initiation (mean and range, SD): < 24 hours
- Method of breeding: Daphnia magna was cultured in glass beakers of 150 mL volume (one parent per vessel) at room temperature 18 – 22o C with the light : dark ratio of 16 h : 8 h (electronic time programmer, KANLUX, Poland). The culture was maintained in Elendt M7 medium [SOP/W/63, SOP/W/67].
- Feeding during test: none

ACCLIMATION
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Pseudokirchneriella subcapitata algae suspension. Group B vitamins and micronutrients necessary for proper growth were supplied with lyophilized Spirulina sp. suspension.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

158 mg/L as CaCO3

Test temperature:

The recorded temperature was in the range of 18.6 – 19.8ºC, constant within ± 0.6ºC

pH:

In the definitive test, the pH values measured at exposure initiation were in the range of 7.83 – 7.99.
At renewal, the pH values measured in old treatments were in the range of 7.83 – 7.85 and in the
range of 7.65 – 7.93 in fresh treatments. The pH values at exposure termination were in the range of
7.70 – 7.80.

Dissolved oxygen:

The dissolved oxygen concentrations measured at exposure initiation were in the range of
7.3 – 8.9 mg/L. At renewal, the dissolved oxygen concentrations measured in old treatments were in
the range of 7.3 – 8.7 mg/L and in the range of 8.2 – 8.8 mg/L in fresh treatments. The dissolved
oxygen concentrations measured at exposure termination were in the range of 8.1 – 8.7 mg/L

Salinity:

64 mg/L as NaHCO3

Nominal and measured concentrations:

loadings: 1000, 455, 207, 94, 43, 19 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers
- Type (delete if not applicable): covered with transparent lids in order to minimize evaporation and to prevent accidental contamination
- Material, size, headspace, fill volume: 150 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16:8 light:dark

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

171 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence interval: 132 – 221

Results with reference substance (positive control):

EC50 = 0.942 mg/L (potassium dichromate)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes