Phenol, 4-isocyanato-, 1,1',1''-phosphorothioate, reaction p roducts with 3-(trimethoxysilyl)-N-(3-(trimethoxysilyl)propy l)-1-propanamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP OECD Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 92/69/EWG, C.4-C (CO2-Entw.); OECD 301-B (1992)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

A mixed population of activated sewage sludge micro-organisms was obtained on
29 January 2007 from the aeration stage of the Severn Trent Water PIc sewage treatment plant at
Loughborough, Leicestershire, UK, which treats predominantly domestic sewage.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

20.4 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

An amount of test material (61.2 mg) was dispersed in approximately 400 ml of culture medium
and subjected to high shear mixing at approximately 7500 rpm for approximately 15 minutes prior
to dispersal in inoculated culture medium. The volume was adjusted to 3 litres to give a final
concentration of 20.4 mg/l, equivalent to 10 mg carboniI.
A test concentration of 10 mg carbonll was employed in the test following the recommendations
ofthe Test Guideline.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

23

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance):
1 % degradation after 1 d
13 % degradation after 2 d
11 % degradation after 3 d
10 % degradation after 6 d
23 % degradation after 8 d
26 % degradation after 10 d
27 % degradation after 14 d
29 % degradation after 16 d
25 % degradation after 20 d
28 % degradation after 22 d
27 % degradation after 24 d
23 % degradation after 28 d

BOD5 / COD results

Results with reference substance:

Points of degradation plot (reference substance):
30 % degradation after 1 d
77 % degradation after 2 d
78 % degradation after 3 d
89 % degradation after 6 d
100 % degradation after 8 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable, not fulfilling specific criteria

Conclusions:

The test material attained 23% degradation after 28 days and therefore cannot be considered to be
readily biodegradable under the strict terms and conditions ofOECD Guideline No 301B.