Dimethylbis[2-[(1-oxohexadecyl)oxy]ethyl]ammonium chloride

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

November 5, 1992- December 3, 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

The test treatments were conducted using single replicates, guideline stipulates duplicate treatments

Justification for type of information:

Refer to section 13 of IUCLID for details on the read-across justification. The study with the read across substance is considered sufficient to fulfil the information requirements as further explained in the provided endpoint summary.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

The test treatments were conducted using single replicates, guideline stipulates duplicate treatments.

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): diethyl ester dimethyl ammonium chloride (E 4607.01)
- Substance type: pure act. ingr. substance
- Physical state: liquid, A 5 % dispersion (E 4607.01) of DEEDMAC was prepared for dosing the test system using the parent test chemical E 4429.01.
- Preparation of Dispersion solution : 5% aqueous dispersion of DEEDMAC made up in 3.7% HCl Solution (pH 2.6)
- Stability under test conditions: yes
- Storage condition of test material: rt

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

The sludge was treated with a mechanical blender and allowed to settle for ~30 minutes. The supernatant was decanted from the settled sludge and used as inoculum at the rate of 10 ml/L of mineral media

Duration of test (contact time):

ca. 28 d

Initial conc.:

ca. 10 mg/L

Based on:

DOC

Initial conc.:

ca. 20 mg/L

Based on:

other: measured TOC of stock solution

Parameter followed for biodegradation estimation:

CO2 evolution

Parameter followed for biodegradation estimation:

TOC removal

Details on study design:

A 301B screening test was run using the following test treattments:
single replicate of the reference compound, Aniline and a single 10 and 20 mg/L test material treatment (E 4607.01)

3L of media was prepared for each treatment as described in the OECD 301 guideline. The inoculum was added to media at a concentration of 10mlL.THe mixture was aerated with CO2 free air for 24 hr to purge the system of CO2. The media containers were connected to 100 ml BaOH2 trapping train. The train is operated by bubbling CO2 free air through the solutions.

The CO2 collected in the BaOH2 traps was titrated with 0.05 N HCL. At the end of the test, the pH of the media was measured and 1 ml of HCL was added to each flask to drive off inorganic carbonate. After overnight incubation, 100 ml sampels were removed for organic carbon analysis.

Reference substance:

aniline

Key result

Parameter:

% degradation (CO2 evolution)

Value:

ca. 76

Sampling time:

28 d

Remarks on result:

other: 10 mg/L conc.

Key result

Parameter:

% degradation (CO2 evolution)

Value:

ca. 79

Sampling time:

28 d

Remarks on result:

other: 20 mg/L conc.

Key result

Parameter:

% degradation (TOC removal)

Value:

ca. 79

Sampling time:

28 d

Remarks on result:

other: 10 mg/L conc

Key result

Parameter:

% degradation (TOC removal)

Value:

ca. 81

Sampling time:

28 d

Remarks on result:

other: 20 mg/L conc.

Details on results:

The two test treatments (10 and 20 mg/L) reached > 60% CO2 production and met the 10 day window ready biodegradation criteria. Reaction products of C16-18/C18 unsaturated fatty acid with methyl diethanolamine, MeCl quaternized can be classified as readily biodegradable.

Results with reference substance:

Aniline (20 mg/L) reached >60% CO2 production by day 11. It passed the current test criteria for a reference substance, > 60% CO2 in < 14 days.

More details on results
Table: Netto CO2 production and % biodegradation ofreaction products of C_16-18/C_{18 unsaturated}fatty acid with methyl diethanolamine, MeCl quaternized (MDEA-Esterquat)

Day of test	10 mg/l		20 mg/l
	mg CO2 cum. netto	biodegrad. %	mg CO2 cum. netto	biodegrad. %
1	- 0.42	- 0.6	0.31	0.2
4	0.0	0.0	2.29	1.7
5	5.04	7.5	6.62	5.0
7	15.14	22.6	29.81	22.3
8	24.55	36.7	49.19	36.8
11	36.99	55.3	71.35	53.3
14	40.79	61.0	82.42	61.6
18	45.19	67.6	92.27	69.0
22	47.99	71.8	99.91	74.7
28	50.74	75.9	106.02	79.3

The test chemical treatments were not conducted in replicate, only as a single analysis. Duplicate analysis and the deviation between these replicates is specified in the current form (1992) of the 301B guideline. However, 2 different concentrations were tested with a similar trend in the results which lends further support to the data. All other criteria of the Guideline were met.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

MDEA-Esterquat C16-18 and C18 unsatd. proved to be readily biodegradable (>60% biodegradation after 28 d) in a study conducted in accordance with the OECD TG 301 B, and in compliance with GLP standards.

Executive summary:

The biodegradation of MDEA-Esterquat C16-18 and C18 unsatd. was studied in accordance with the OECD TG 301 B, and in compliance with GLP standards for 28 d. MDEA-Esterquat C16-18 and C18 unsatd. was applied at 2 concentration of 10 and 20 mg/l. CO2 production was analysed at 0, 1, 4, 5, 7, 8, 11, 14, 18, 22, and 28 days of incubation.The two test treatments (10 and 20 mg/L) reached > 60% CO2 production and met the 10 -day window. Therefore MDEA-Esterquat C16-18 and C18 unsatd. fulfills the OECD criteria of ready biodegradability.

Description of key information

The biodegradation of MDEA-Esterquat C16-18 and C18 unsatd. was studied in accordance with the OECD TG 301 B, and in compliance with GLP standards for 28 d. MDEA-Esterquat C16-18 and C18 unsatd. was applied at 2 concentration of 10 and 20 mg/l. CO2 production was analysed at 0, 1, 4, 5, 7, 8, 11, 14, 18, 22, and 28 days of incubation.The two test treatments (10 and 20 mg/L) reached > 60% CO2 production and met the 10 -day window. Therefore MDEA-Esterquat C16-18 and C18 unsatd. fulfills the OECD criteria of ready biodegradability.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater