Registration Dossier
Registration Dossier
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EC number: 618-333-0 | CAS number: 9001-85-8
Endpoint summary
Administrative data
Description of key information
The short-term toxicity of lysophospholipase to fish and crustacean was not conducted, however, an enzyme (IUB 3.1.1.3) from the same sub-sub-class was used for read-across.
Cutinase was tested on rainbow trout and the 96 hour LC50 value was > 68.3 mg active enzyme protein (aep)/L and the no-observed effect concentration 11.2 mg aep/L. Cutinase was also tested on invertebrate Daphnia magna and no immobilization was seen in concentrations up to 37.4 mg aep/L, as well as on blue green algae and did not inhibit the growth in concentration up to 41.3 mg aep/L.
Lipolase was tested on blue green algae and the no observed effect concentration was 7.4 mg aep/L. The 72h EbC50 is 17.6 mg aep/L and the ErC50 is 18 mg aep/L.
Given the similarity between the enzymes, it is expected that lysophospholipase will have similar short-term toxicity values to fish, invertebrates and algae.
Inhibition control carried out in the test of ready biodegradability showed no inhibition of the activated sludge inoculum at an enzyme concentration above the expected levels in inlet to sewage treatment plants (STPs). Monitoring of enzymes in the inlet to municipal STPs (in Denmark) resulted in concentrations of less than 2 µg aep/L which are below the initial concentration used in tests for ready biodegradability, where no inhibitory effects were observed. It is concluded that a study on activated sludge respiration inhibition does not need to be conducted. Therefore, lysophospholipase is not considered to be toxic to microorganisms.
Additional information
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