Reaction products of diazotised 3-amino-2-hydroxy-5-nitrobenzenesulphonic acid and 3-amino-4-hydroxy-5-nitrobenzenesulphonic acid, coupled with resorcinol, metalized with Ferric Chloride, condensed with Formaldehyde, sodium salts

Endpoint summary

Description of key information

Short-term toxicity to fish

LC50 (Leuciscus idus L., 96 h) 31.66 mg/L

Short-term toxicity to invertebrates

EC50 (Daphnia magna, 48 h) = 1.03 mg/L

Toxicity to algae and cyanobacteria

IC50 ( Pseudokirchneriella subcapitata, 72 h) = 3.4 mg/L

Additional information

Short-term toxicity to fish

The substance was tested for acute toxicity following the guideline DIN 38 412 part 15 (1982). Ten fishes Golden orfe (Leuciscus idusL. Golden variety) were exposed in a static system at the nominal concentrations of: 4.64, 10, 21.5, 46.4 and 100 mg/L.

Toxicity to microorganisms

The toxicity to microorganisms was evaluated in a test performed following the method DIN 38412 part 27 (1989). Pseudomonas putida microorganisms were exposed at ten concentrations from 39.1 to 10000 mg/L in a static system. The EC 50 (Pseudomonas putida, 30 min) was found to be ≥ 10000 mg/L.

The following data was obtained for Similar Substance 01 on Daphnia magna and Pseudokirchneriella subcapitata, respectively. It is expected that the Target Substance will present similar effect levels to these aquatic organisms. Justification for the use of a read-across approach is provided in Section 13 of IUCLID.

Freshwater Crustacea assessment

The substance has been tested for short-term toxicity on freshwater invertebrates according to the guideline OECD 202 (1984). The organisms have been exposure to the test item at concentrations of 0.1 mg/l, 1 mg/l, 10 mg/l, 100 mg/l and 1000 mg/l for 48 hours. 

The EC50(48 h) was determined to be 1.03 mg/l.

Freshwater Alga and Cyanobacteria assessment

The substance has been tested for acute toxicity on algae according to the OECD guideline 201 (1984). The alga Pseudokirchneriella subcapita has been subjected to the substance at the test concentrations of 0.1 mg/l, 0.32 mg/l, 1 mg/l, 3.2 mg/l, 10 mg /l, 32mg/l and 90mg/l for 72 hours.

Justification for classification or non-classification

According to the CLP Regulation (EC n. 1272/2008), Part 4: Environmental Hazards, the substances can be classified for hazardous to the aquatic environment when the following criteria are met:

A )Acute (short-term) aquatic hazard Category Acute 1: 96 hr LC 50 (for fish) and/or 48 hr EC 50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/l.

B) Long-term aquatic hazard (iii) Substances for which adequate chronic toxicity data are not available and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log K ow ≥ 4).

Category Chronic 1: 96 hr LC 50 (for fish) and/or 48 hr EC 50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) ≤ 1 mg/L

Category Chronic2: 96 hr LC 50 (for fish) and/or 48 hr EC 50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants)> 1 to ≤10 mg/L

Category Chronic 3:96 hr LC 50 (for fish) and/or 48 hr EC 50 (for crustacea) and/or 72 or 96 hr ErC 50 (for algae or other aquatic plants) > 10 to ≤ 100 mg/L.

 

The substance is not rapidly degradable and the available short term toxicity test to intertebrates and algae determined the effect level that meets the criteria related to the hazard Category Chronic 2 (> 1 to ≤ 10 mg/L). Therefore, the substance is classified as Aquatic Chronic 2 according to the CLP Regulation (EC) No. 1272/2008.