Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Remarks:

Not GLP study

Test material

Test material information

Constituent 1

Reference substance name:

Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

EC Number:

304-762-8

EC Name:

Tetrasodium 4-[[3,5-bis[[4-[(4-nitro-2-sulphonatophenyl)amino]phenyl]azo]-2,4(or 2,6)-dihydroxyphenyl]azo]-5-hydroxynaphthalene-2,7-disulphonate

Cas Number:

94279-16-0

Molecular formula:

C40H24N10Na4O19S4

IUPAC Name:

tetrasodium 4-[(1E)-2-{2,6-dihydroxy-3,5-bis[(1E)-2-{4-[(4-nitro-2-sulfonatophenyl)amino]phenyl}diazen-1-yl]phenyl}diazen-1-yl]-5-hydroxynaphthalene-2,7-disulfonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia Magna, Strauss (Cladocera, Crustacea)
TEST ORGANISM
- Strain:Daphnia Magna, Strauss (Cladocera, Crustacea)
- Source: Stirling University, UK
- Age at study initiation (mean and range, SD): <= 24h lifetime

ACCLIMATION
- Food type: Chlorella (freshwater algae) and marine algae extract
- Frequency: 3 times/week
-Temperature: 18-22°C
- Photoperiod: 16 light/8 darkness
- without areation
- Health during acclimation (any mortality observed): no

TEST period
-Temperature: 20 +/- 2°C
-Photoperiod: darkness

Study design

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

pH:

7.73-7.95

Dissolved oxygen:

7.92-8.17 mg/l

Salinity:

CaCl2 2H2O= 11.76 mg/l
MgSO4 7H2O = 4.93 mg/l
NaHCO3= 2.59 mg/l
KCl= 0.23 mg/l

25 ml of each solution were added to 1000 ml deionised water

Nominal and measured concentrations:

0, 0.1, 1, 10 and 100 and 1000 mg/l of active ingredient (dye)

Details on test conditions:

TEST SYSTEM
- Test vessel: tubes of 40 ml sealed with parafilm
- Type (delete if not applicable): closed
- Aeration: no areation
- No. of organisms per vessel: 5 (for a total of 20)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control vehicle (replicates): 4

Reference substance (positive control):

yes

Remarks:

K2Cr2O7

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 31.6 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 17.8 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Results with reference substance (positive control):

- EC50/LC50: 24h, 0.86 mg/l

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance was tested for acute toxicity on Daphnia (Immobilisation) following OECD 202. The tested sample under the experimental conditions showed an EC50,48h = 17.8 mg/l

Executive summary:

The substance was tested for acute toxicity on Daphnia (Immobilisation) following OECD 202. The test concentraions were 0.1, 1, 10, 100 and 1000 mg/l of dye for a total exposure of 48h. Vehicle and positive controls were also performed fulfilling validity criteria for the test. The tested sample under the experimental conditions showed an EC50,48h = 31.6 mg/l.

The substance showed toxic effects to daphnia and needs to be classified under Regulation 1272/2008 (CLP) as Aq. Chronic 3, H412.