Ethanol, 2-(ethenylthio)-

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1967-09-26 to 2016-10-09

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1967

Report date:

1967

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

according to BASF-internal standard
The test substance was administered to mice by i.p. injection.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethanol, 2-(ethenylthio)-

EC Number:

608-554-0

Cas Number:

3090-56-0

Molecular formula:

C4H8OS

IUPAC Name:

Ethanol, 2-(ethenylthio)-

Test material form:

liquid

Specific details on test material used for the study:

No specific details for test material were provided.

Test animals

Species:

mouse

Strain:

other: Kisslegg

Sex:

male/female

Details on test animals or test system and environmental conditions:

No details were provided.

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: water emulsion containing Traganth

Details on exposure:

- Application form: emulsion
- Concentration in vehicle: 2, 4, 8 and 20 % (v/v)

Doses:

0.2, 0.4, 0.5, 0.64, 0.8 and 1.6 mL/kg
213.38, 426.76, 533.45, 682.82, 853.52 and 1707.04 mg/kg (calculated from mL/kg with a density of 1.0669 g/cm^3)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

No statistical analysis was performed.

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

0.57 mL/kg bw

Based on:

test mat.

Effect levels 2

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

608.13 mg/kg bw

Based on:

test mat.

Mortality:

1.6 mL/kg: All 10 animals died within 24 h after application.
0.8 mL/kg: 9 animals died within 24 h after application. 1 animal died within 48 h after application.
0.64 mL/kg: 6 animals died within 24 h after application. 2 animals died within 48 h after application. 1 animal died between 48 h and 7 days after application.
No further mortality was observed.

Clinical signs:

Directly after application animals started to show forced breathing, dragging of hind legs, shaking, staggering walk, spasms, ventral position, partially anesthetic like behavior, closed eyes and dyspnea. Survivors presented with unchanged symptoms for 1 day. Afterwards animals recovered and were without symptoms after 3-5 days.

Body weight:

Body weights were not determined.

Gross pathology:

In 5 animals that died after treatment weak agglutinations of liver and surrounding organs was detected. In sacrificed animals 11 weak agglutinations and 3 strong adhesions of liver and surrounding organs were detected.
No other observations were made in survivors or animals that died after treatment.

Other findings:

No other observations were made.