Phenoxazin-5-ium, 3,7-bis(diethylamino)-, formate (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 - 31 Oct 1985

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)

Version / remarks:

1982

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phenoxazin-​5-​ium, 3,​7-​bis(diethylamino)​-​, formate (1:1)

EC Number:

601-264-5

Cas Number:

113534-44-4

Molecular formula:

C21H27N3O3

IUPAC Name:

Phenoxazin-​5-​ium, 3,​7-​bis(diethylamino)​-​, formate (1:1)

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

Stock solution (1 g/L) prepared and diluted with dilution water to final test concentrations

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Source: Institut National de Recherche Chimique Appliquee, France, bred at BASF AG since 1978

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.67 mmol/L

Test temperature:

20°C

pH:

7.9 - 8.3

Dissolved oxygen:

8.5 - 9.3 mg/L

Conductivity:

560 µS/cm

Nominal and measured concentrations:

Nominal: 0 (control), 0.039, 0.078, 0.156, 0.313, 0.625, 1.25, 2.50, 5.00, 10.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beaker (250 mL total volume)
- Material, size, headspace, fill volume: 200 mL fill volume
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 40 mL/animal

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 light / 8 darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility at 0, 3, 6, 24, 48 h

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.576 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CI: 0.443 - 0.708 mg/L

Details on results:

0% mortality in control

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-h EC50 to Daphnia was determined at 0.576 mg/L (nominal).