N,N'-bis(3-aminopropyl)piperazine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016-10-21 - 2017-01-23

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-bis(3-aminopropyl)piperazine

EC Number:

230-589-1

EC Name:

N,N'-bis(3-aminopropyl)piperazine

Cas Number:

7209-38-3

Molecular formula:

C10H24N4

IUPAC Name:

3-[4-(3-aminopropyl)piperazin-1-yl]propan-1-amine

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Activated sludge from the municipal sewage treatment plant , 31137 Hildesheim, Germany. The activated sludge from the sewage treatment plant Hildesheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
The activated sludge was washed twice with chlorine free tap water. Afterwards the settled sludge was resuspended in mineral salt medium and was maintained in aerobic condition by aeration for two hours. Thereafter the sludge was homogenized with a blender. After sedimentation the supernatant was decanted and maintained under aeration with CO2-free air for 6 days.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

25 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

0

Sampling time:

28 d

Details on results:

Both two test item replicates did not reach the 10% biodegradtion level (beginnin of biodegradation) within the 28 days test period. The mean biodegradation was 0%.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

Both two test item replicates did not reach the 10% biodegradtion level (beginnin of biodegradation) within the 28 days test period. The mean biodegradation was 0%. The test item is classified a not readily biodegradable within the 28 days period of the study.

Executive summary:

The ready biodegradability of the test item N,N`-bis(3-aminopropyl)piperazine was determined with non-adapted activated sludge in the Manometric Respirometry Test for a period of 28 days. The study was conducted with the definite exposure phase from 2016 -11 -16 to 2016 -12 -14, according to OECD guideline 301 F at the test facility. The test item concentration selected as appropriate was 25.0 mg/L, corresponding to a ThOD of 52.0 mg O₂/L (83.8 mg O₂/L with nitrification) per test vessel. The oxygen was depleted by the respiration of bacteria and the degradation was followed by measuring the oxygen concentration. The biodegradation rate is therefore expressed as the percentage BOD (biological oxygen demand) and was calculated for each study day.

 

The mean oxygen depletion in the inoculum control was 9.60 mg O₂/L on day 28.

 

In order to check the activity of the test system sodium benzoate was used as functional control. The pass level > 60% was reached on day 3. The biodegradation reached a maximum of 98% degradation on day 27.

 

In the toxicity control containing both test and reference item 51% degradation occurred within 14 days. After 28 days the biodegradation reached 54%. The degradation of the reference item was not inhibited by the test item.

Both test item replicates did not reach the 10% level (beginning of biodegradation) within the 28 days period of the study. The mean biodegradation on day 28 was 0%.