ethametsulfuron-methyl (ISO); methyl 2-[(4-ethoxy-6-methylamino-1,3,5-triazin-2-yl)carbamoylsulfamoyl]benzoate

Toxicity to birds

Administrative data

Endpoint:

short-term toxicity to birds: acute oral toxicity test

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EPA OPP 71-1 (Avian Acute Oral Toxicity Test)

Deviations:

yes

Remarks:

Verification of test conc., stability, and homogeneity in diluent not determined. Periodic analyses of water and feed for potential contaminants not performed according to GLP standards (done at certified laboratory and standard US EPA analytical methods)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.2100 (Avian Acute Oral Toxicity Test)

Deviations:

yes

Remarks:

Verification of test conc., stability, and homogeneity in diluent not determined. Periodic analyses of water and feed for potential contaminants not performed according to GLP standards (done at certified laboratory and standard US EPA analytical methods)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Reference substance 002

Cas Number:

97780-06-8

Test material form:

solid

Details on test material:

- Purity: 99.2%

Dose method:

gavage

Analytical monitoring:

no

Vehicle:

yes

Remarks:

1% CMC aqueous solution

Test organisms

Test organisms (species):

Colinus virginianus

Study design

Post exposure observation period:

14 days

No. of animals per sex per dose and/or stage:

5

Control animals:

yes, concurrent vehicle

Nominal and measured doses / concentrations:

Nominal: 0, 292, 486, 810, 1350, and 2250 mg a.s./kg bw

Examinations

Reference substance (positive control):

no

Results and discussion

Effect levels

Effect levels 1

Key result

Dose descriptor:

LD50

Effect level:

> 2 250 mg/kg bw

Conc. / dose based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: highest concentration tested

Effect levels 2

Key result

Dose descriptor:

NOEL

Effect level:

2 250 mg/kg bw

Conc. / dose based on:

act. ingr.

Basis for effect:

signs of toxicity

Remarks on result:

other: highest concentration tested

Applicant's summary and conclusion

Conclusions:

LD50 (Northern bobwhite): >2250 mg a.s./kg bw (highest concentration tested)
NOEL (Northern bobwhite): 2250 mg a.s./kg bw (highest concentration tested)

Executive summary:

The test substance was administered to fasted northern bobwhite quail (Colinus virginianus) in an acute oral toxicity study. The study was conducted according to EPA OPP 71-1 and EPA OPPTS 850.2100. Five male and five female bobwhites per dose received single oral nominal doses of 0, 292, 486, 810, 1350, and 2250 mg a.s./kg bw at a dose volume of 10 mL/kg bw in 1% CMC aqueous solution. Birds were observed for clinical signs of toxicity, body weight effects, and mortality for 14 days after dosing. Since there were no mortalities or signs of toxicity, no birds were examined for gross pathological changes. The acute oral LD50 value for northern bobwhite exposed to the test substance by single oral dose was >2250 a.s. /kg bw, the highest concentration tested. The no mortality and no-observed effect level was 2250 mg a.s./kg bw.