6-methoxynaphthalene-2-carbaldehyde

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from NTRL report

Data source

Reference

Reference 1

Reference Type:

secondary source

Title:

FINAL REPORT OF SEVERAL STUDIES ON VANILLIN WITH COVER LETTER DATED 112191

Author:

NTRL (National Technical Reports Library)

Year:

1991

Bibliographic source:

NTRL (National Technical Reports Library); OTS05343EA; 22 NOVEMBER 1991

Reference 2

Reference Type:

secondary source

Title:

SIDS INITIAL ASSESSMENT REPORT- Vanillin

Author:

OECD SIDS

Year:

1996

Bibliographic source:

SIDS INITIAL ASSESSMENT REPORT- Vanillin; OECD SIDS; 28/10/1996.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

An eye irritation test of Vanillin (CAS No: 121-33-5) was performed in six New Zealand White rabbits to assess its eye irritation potential.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Vanillin

EC Number:

204-465-2

EC Name:

Vanillin

Cas Number:

121-33-5

Molecular formula:

C8H8O3

IUPAC Name:

1-butoxypropan-2-ol

Test material form:

solid

Details on test material:

- Name of test material (as cited in study report): Vanillin
- Molecular formula: C8H8O3
- Molecular weight: 152.1482 g/mol
- Substance type: organic
- Physical state: Solid
-Smiles: c1(cc(ccc1O)C=O)OC
-InChI: 1S/C8H8O3/c1-11-8-4-6(5-9)2-3-7(8)10/h2-5,10H,1H3

Specific details on test material used for the study:

- Name of test material (as cited in study report): Vanillin
- Molecular formula: C8H8O3
- Molecular weight: 152.1482 g/mol
- Substance type: organic
- Physical state: Solid
-Smiles: c1(cc(ccc1O)C=O)OC
-InChI: 1S/C8H8O3/c1-11-8-4-6(5-9)2-3-7(8)10/h2-5,10H,1H3

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand Black

Details on test animals or tissues and environmental conditions:

No data available

Test system

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

500mg (0.1ml)

Duration of treatment / exposure:

24 hours

Observation period (in vivo):

24, 48 and 72 hours

Number of animals or in vitro replicates:

6

Details on study design:

SCORING SYSTEM: Federal Hazardous Substances Act (FHSA)

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

24/48/72 h

Score:

18.8

Max. score:

110

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Irritant / corrosive response data:

Mild irritating effects were observed.

Any other information on results incl. tables

EYE IRRITATION SCORES
Hours	1			24			48			72			120			168
Structure	Cr	Ir	Con	Cr	Ir	Con	Cr	Ir	Con	Cr	Ir	Con	Cr	Ir	Con	Cr	Ir	Con
Animal no.
1	0	0	10	10	5	14	5	5	12	0	0	8	0	0	2	0	0	0
2	0	0	10	5	0	14	5	0	12	0	0	8	0	0	2	0	0	0
3	0	0	14	20	5	16	20	5	12	10	0	8	5	0	4	0	0	0
4	0	0	10	5	0	12	5	0	12	0	0	8	0	0	2	0	0	0
5	0	0	10	10	5	16	10	5	12	0	0	8	0	0	4	0	0	0
6	0	0	10	5	0	10	5	0	10	0	0	2	0	0	0	0	0	0
Mean score	10.6			25.3			22.5			8.6			3.1			0.0
Cr- cornea, Ir- iris,  Con-Conjunctivae
Immediate: Discomfort was moderate with eyes tightly closed Comments: 10 min: Slight to moderate erythema; very slight to slight edema 1 Hr.: slight to moderate erythema ; very slight to slight edema 24 Hr.: area of barely perceptible corneal dullness, iris reaction to light was sluggish, moderate to severe erythema very slight to slight edema, copious discharge containing much whitish exudates. 48 - 120 Hr.: Gradually improvement. 168 Hr.: All scored zero.

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

The chemical Vanillin (CAS No: 121-33-5) was considered to be mildly irritating to the eyes of six New Zealand White rabbits.

Executive summary:

An eye irritation test of Vanillin (CAS No: 121-33-5) was performed in six New Zealand White rabbits to assess its eye irritation potential.

 

The Vanillin was applied as finely ground powder at a dose equivalent to 55 mg (0.1 ml) for 24 hours and reactions were scored at 24 , 48 and 72 hours according to Federal Hazardous Substances Act (FHSA).

 

Mild irritating effects were observed with the irritation score of 18.8 (out of maximum score of 110). All scored zero after 168 hours.

 

Since the chemical caused slight discomfort and ocular lesions during the 72 hours observation period, the chemical Vanillin (CAS No: 121-33-5) was considered to be mildly irritating to the eyes of six New Zealand White rabbits