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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Bioaccumulation: terrestrial

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Administrative data

Endpoint:
bioaccumulation: terrestrial
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study meets generally accepted scientific standards, well documented and acceptable for hazard assessment

Data source

Reference
Reference Type:
publication
Title:
Metal accumulation strategies in saprophagous and phytophagous soil invertebrates: a quantitative comparison.
Author:
Gräff S, Berkus M, Alberti G, Köhler HR
Year:
1997
Bibliographic source:
Biometals 10(1): 45-53.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Calculation of BSAF values using terrestrial isopods which were exposed for 3 weeks to lead containing decomposed leaf litter.
GLP compliance:
not specified

Test material

Constituent 1
Reference substance name:
Lead dichloride
EC Number:
231-845-5
EC Name:
Lead dichloride
Cas Number:
7758-95-4
Constituent 2
Reference substance name:
007758-95-4
Cas Number:
007758-95-4
IUPAC Name:
007758-95-4
Details on test material:
No further information on test material is stated.
Radiolabelling:
no

Test substrate

Vehicle:
no

Test organisms

Test organisms (species):
Porcellus scaber
Details on test organisms:
Adults of Porcellio saber were supplied from a laboratory hatchery.

Test conditions

Test temperature:
15°C
pH:
not reported
TOC:
not reported
Moisture:
not reported
Details on test conditions:
-laboratory experiment
- Organisms were exposed to partly decomposed leaf litter material soaked in an aqueous solution of 100, 500 or 1000mg/kg Pb2+ (as PbCl2), or 500, 1000 for three weeks. Controls were wetted with tap water. Isopods were kept in plastic boxes on a moist gypsum base covered by the above mentioned partly decomposed leaf litter material; To adjust exposure conditions to the respective preferences of the test species and to meet the field situation.



Nominal and measured concentrations:
measured lead concentrations (mg Pb/kg dw): 7.1 (control), 517.1, 2777.5 and 7676.0

Results and discussion

Bioconcentration factoropen allclose all
Type:
BSAF
Value:
0.41 other: kg dw / kg dw
Basis:
whole body d.w.
Calculation basis:
steady state
Remarks on result:
other: duration: 21 days; organism: Porcellio scaber; substrate: control
Type:
BSAF
Value:
0.14 other: kg dw / kg dw
Basis:
whole body d.w.
Calculation basis:
steady state
Remarks on result:
other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 100 mg Pb/L
Type:
BSAF
Value:
0.08 other: kg dw / kg dw
Basis:
whole body d.w.
Calculation basis:
steady state
Remarks on result:
other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 500 mg Pb/L
Type:
BSAF
Value:
0.03 other: kg dw / kg dw
Basis:
whole body d.w.
Calculation basis:
steady state
Remarks on result:
other: duration: 21 days; organism: Porcellio scaber; substrate: litter soaked in 1000 mg Pb/L
Reported statistics:
Student's t-test; p<0.001 (higly significant)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Results can be used in a weight of evidence approach to calculate bioaccumulation factors in soil.