Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 217-968-7 | CAS number: 2022-85-7
Adsorption / desorption
Administrative data
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-06-07 - 2004-07-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- 22 January 2001.
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- other: please refer to details of method
Test material
- Reference substance name:
- Flucytosine
- EC Number:
- 217-968-7
- EC Name:
- Flucytosine
- Cas Number:
- 2022-85-7
- Molecular formula:
- C4H4FN3O
- IUPAC Name:
- flucytosine
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- please refer to Test material information
- Radiolabelling:
- no
Study design
- Test temperature:
- Column temperature: 30°C
HPLC method
- Details on study design: HPLC method:
- EQUIPMENT
- Apparatus: Agilent Technologies 1100, incorporating autosampler and workstation
- Type, material and dimension of analytical (guard) column: Phenosphere-NEXT 5µ CN (250 x 4.6 mm id)
- Detection system: UV detector, wavelength: dead time and reference standards: 210 nm; sample: 260 nm
MOBILE PHASES
- Type: methanol:water (55:45 v/v)
- pH: 7.0
- Solutes for dissolving test and reference substances: methanol
DETERMINATION OF DEAD TIME
- Method: The dead time was determined by measuring the retention time of formamide (purity* ≥99.5%, 685 mg/L solution in methanol). *value quoted by supplier
REFERENCE SUBSTANCES
Standard Purity (%)* Concentration (mg/L)
Acetanilide ~99 106
Phenol 99.99 223
Atrazine 99.2 100
Isoproturon 99.9 200
Triadimenol 98.5 146
Linuron 99 115
Naphthalene ≥99.0 109
Endosulfan-diol 99.9 101
Fenthion 96 144
α-Endosulfan 99.8 109
Phenanthrene ≥97.0 104
Diclofop-methyl 99.3 107
DDT 98 253
DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: Injection volume: 10 µL
- Quantity of reference substances: Injection volume: 10 µL
REPETITIONS
- Number of determinations: The sample, formamide and reference standard solutions were injected in duplicate
EVALUATION
- Calculation of capacity factors k': The capacity factor was calculated using: k = (tr-t0)/t0 ; where: k=capacity factor, r=retention time (min), t0=dead time (min)
- Determination of the log Koc value: A calibration curve was constructed from the retention time data of the formamide and reference standard solutions; the log10 Koc value was determined with reference to the calibration curve (Fig. 12.1 of attached study report)
Results and discussion
Adsorption coefficientopen allclose all
- Key result
- Sample No.:
- #1
- Type:
- log Koc
- Value:
- < 1.25 dimensionless
- pH:
- 7
- Temp.:
- 30 °C
- Matrix:
- column matrix
- Key result
- Sample No.:
- #2
- Type:
- log Koc
- Value:
- < 1.25 dimensionless
- pH:
- 7
- Temp.:
- 30 °C
- Matrix:
- column matrix
- Key result
- Type:
- Koc
- Value:
- < 17.8 dimensionless
- pH:
- 7
- Temp.:
- 30 °C
- Matrix:
- column matrix
Results: HPLC method
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration:
Standard / Mean Retention time (mins)
Acetanilide / 3.936
Phenol / 3.878
Atrazine / 4.619
Isoproturon / 4.909
Triadimenol / 5.903
Linuron / 6.042
Naphthalene / 6.549
Endosulfan-diol / 7.443
Fenthion / 9.209
α-Endosulfan / 15.318
Phenanthrene / / 11.069
Diclofop-methyl / 14.233
DDT / 43.265
- Average retention data for test substance: 3.274 mins
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The adsorption coefficient (Koc) of the test material has been determined to be <17.8, log10 Koc <1.25.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
