Pyrolysis light oil from waste plastics

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Specific details on test material used for the study:

Name Naphtha/Light Oil
Batch no. 19088.09
Appearance clear, yellow liquid
Composition Aromatic hydrocarbons 20 - 45 %; Naphthenic hydrocarbons 20 - 45 %; Olefinic hydrocarbons 10 - 30 %; Paraffinic hydrocarbons 10 - 30 %; Styrene 0 - 10 %; Toluene 0 - 10 %; Ethylbenzene 0 - 10 %; Benzene ≥ 0.1 %
CAS No. not assigned
EC-No. 940-514-0
Molecular formula not stated
Molecular weight ca. 113 g/mol
Purity 100 % UVCB
Homogeneity homogeneous
Vapour pressure not stated
Stability H2O: not stated; EtOH: not stated; acetone: not stated; CH3CN: not stated; DMSO: not stated
Solubility H2O: not stated; EtOH: not stated; acetone: not stated; CH3CN: not stated; DMSO: not stated
Expiry date 31. Dec. 2025
Storage Room Temperature (20 ± 5 °C)

Preparation of the test item prior to test
Experiment 1:
The water-accommodated fractions (WAFs) were prepared for the test. This was done by pipetting the nominal loads of 46 / 100 / 220 / 460 / 1000 mg/L resp. 62.2 / 135.3 / 297.7 / 622.5 / 1353.2 µL/L test item (based on a density of 0.739 g/mL given by the SDS) of the corresponding amount of dilution water and stirring vigorously for 24 hours. The resulting solution was used unfiltrated.
Experiment 2:
The water-accommodated fractions (WAFs) were prepared for the test. This was done by pipetting the real loads of 4.6 / 10 / 22 / 46 / 100 mg/L resp. 6.2 / 13.5 / 29.8 / 62.3 / 135.3 µL/L test item (based on a density of 0.739 g/mL given by the SDS) of the corresponding amount of dilution water and stirring vigorously for 23 hours and 50 minutes. The result-ing solution was used unfiltrated.

Vehicle:

no

Details on test solutions:

Dilution water (Daphnia medium) with the following specification was used (stated in SOP
Parameter Concentration in mg/L
CaCl2*2H2O 293.80
MgSO4*7H2O 123.30
NaHCO3 64.80
KCl 5.80

Test organisms (species):

Daphnia magna

Details on test organisms:

Young daphnia, aged less than 24 hours
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007
Acclimatation
Vessels preserving glasses, nominal volume 2 L
Medium M4-Medium (recipe of ELENDT)
Food green algae (Desmodesmus subspicatus)
Medium renewal twice a week
Photo period 16/8 hours, using neon tubes
Temperature 20  2 °C

Test type:

static

Water media type:

brackish water

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Immobilisation was recorded at 24 hours and 48 hours

Test temperature:

19.4 – 20.9 °C Experiment 1
19.0 – 20.5 °C Experiment 2

pH:

Experiment 1: 7.4-7.7
Experiment 2: 7.5-7.6

Dissolved oxygen:

Experiment 1: 7-7.7 mg/l
Experiment 2: 7.9-8.9 mg/l

Nominal and measured concentrations:

Experiment 1: 46 / 100 / 220 / 460 / 1000 mg/L (nominal concentration)
Experiment 2: 4.6 / 10 / 22 / 46 / 100 mg/L (nominal concentration)

Details on test conditions:

Treatments
Experiment 1:
46 / 100 / 220 / 460 / 1000 mg/L (nominal concentration)
The concentrations to be tested are based on the result of a non-GLP pre-test. In the pre-test open glass beakers were used, but the first experiment was performed in closed glass flasks, because of the volatile character of the test item. Therefore, the chosen concentrations in the first experiment were too high.
Experiment 2:
4.6 / 10 / 22 / 46 / 100 mg/L (nominal concentration)
The concentrations to be tested are based on the result of Experiment 1.
Temperature 19.4 – 20.9 °C Experiment 1
19.0 – 20.5 °C Experiment 2
Duration: 48 hours
Observation times: 24 and 48 hours
Medium renewal: none
Test vessel: Closed glass flasks nominal volume 65 mL
Treatments 4 vessels, each completely filled with test solution and 5 daphnia
Blank control 4 vessels, each completely filled with dilution water and 5 daphnia

Reference substance (positive control):

yes

Remarks:

Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 48.43 mg/L

95% CI:

> 39.41 - < 59.95

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 36.09 mg/L

95% CI:

> 28.83 - < 45.32

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

ca. 22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Details on results:

All tested concentrations (46, 100, 220, 460, 1000 mg/l) showed a toxicity of 100 % immobilisation after 48 hours. None of the animals was immobilised in the blank control.
In the blank control, none of the daphnia was immobilised

Validity criteria fulfilled:

yes

Conclusions:

48h-NOEC = 10 mg/L
48h-LOEC = 22 mg/L
24h-EC50 = 48.43 mg/L
48h-EC50 = 36.09 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

ca. 36.09 mg/L