bis[2-(2-butoxyethoxy)ethyl] succinate

Toxicity to aquatic algae and cyanobacteria

Description of key information

Algal biomass and growth inhibition (species: Pseudokirchneriella subcapitata) is evaluated during 72 hours of exposure to bis(2-(2-butoxyethoxy)ethyl)butanedionate, using number of cells as growth indicator. Test was performed according to OECD TG 201 Test concentrations were 12.5, 25, 50, 100 and 200 mg/l and no solvent was used.

Key value for chemical safety assessment

EC50 for freshwater algae:

200 mg/L

EC10 or NOEC for freshwater algae:

100 mg/L

Additional information

The effect of bis(2-(2-butoxyethoxy)ethyl)butanedionate on growth of the unicellular algal speciesPseudokirchneriella subcapitatawas measured during a 72 hours exposure period. Based on the results from a range finding study, the main study was conducted at12.5, 25, 50, 100 and 200mg/L  bis(2-(2-butoxyethoxy)ethyl)butanedionate  in OECD TG 201 medium. No solvent was used. Results are expressed in geo mean values. The test was performed in triplicate, at 21 to 24°C, pH 7.3-7.7and light intensity of approximately 60-120 µEm-2 S-1. The medium composition is reported. At 0, 24, 48 and 72 hours the number of cells was measured in each test vessel (hemacytometer). Results were expressed as % growth inhibition compared to the control, for biomass and growth inhibition. The effect concentrations and the 95% confidence levels were calculated. Information to evaluate the test quality is available and the pH did not shift more than 1 unit. Test results are therefore valid. Analytical results are documented for concentration of the test substance during the exposure. The E_rC₅₀is >200 mg/l and E_yC₅₀is 198 mg/l.

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