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EC number: 605-296-0 | CAS number: 162627-17-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- Quality Assurance Programme/GLP standards not specified
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: water accommodated fraction (WAF)
A saturated stock solution of the test material with an initial weight of 270 mg was prepared in 500 ml of water for injection and stirred overnight at 20 °C to 25 °C. After a filtration step over a sterile membrane filter (Minisart NML, Sartorius, Göttingen, Germany, pore size 0.2 µm) the solution was used in the test.
- Controls: water for injection - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: Pseudomonas putida DSM 50026, Stamm Berlin 33/2 obtained from the German collection of microorganisms (DSM, 38124 Braunschweig, Germany)
- Method of cultivation: Stock cultures were stored in a freezer at -80 °C in test tubes with Casein soya bean digest broth containing 7.5 % DMSO. The working culture of the strain Pseudomonas putida was stored in test tubes in solid nutrient medium. For the preservation of the test strain new strain cultures were made at one week intervals. These cultures were incubated and stored at 25 °C.
- Preparation of inoculum for exposure: The material of inoculation for the precultures was taken from a working culture up to 7 days old. One day prior to the start of the experiment the inoculation was multiplied in liquid medium. This grown preculture was diluted with medium in a way that a defined turbidity of FAU (Formazine Attenuation Units) = 10 was yielded arithmetically, e. g. by addition of 10 ml of a bacterial suspension with a turbidity of FAU = 100 to 90 ml of medium. The preculture was incubated for 7 hours at 21 °C ± 1 °C. After the incubation period the bacterial suspension was diluted with culture medium so that a defined turbidity of FAU = 50 was yielded arithmetically.
- Initial biomass concentration: FAU = 5 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 16 h
- Test temperature:
- 21°C +/- 1 °C
- Nominal and measured concentrations:
- nominal loading rate: 430 mg/L
DOC-value of the test solution at test start: 1.5 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 300 mL Erlenmeyer flasks, fill volume 100 mL
- Bacterial starting concentration: FAU = 5
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: nutrient solution containing dextrose (2 g/L)
- Culture medium different from test medium: The culture medium contained supplemental yeast extract (50 mg/L)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): growth inhibition measured at test end - Key result
- Duration:
- 16 h
- Dose descriptor:
- IC50
- Effect conc.:
- >= 430 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: results are expressed in terms of loading rates
- Key result
- Duration:
- 16 h
- Dose descriptor:
- IC10
- Effect conc.:
- >= 430 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Remarks on result:
- other: results are expressed in terms of loading rates
- Details on results:
- In a saturated solution of the test material (a nominal loading rate 430 mg/L) no significant inhibition effects on the test organisms were observed.
Reference
Table 1: Formazine Attenuation Units (FAU) at test end (16 ± 1 h)
Nominal loading rate (mg/l) |
Formazine Attenuation Units (FAU/436 nm) |
Inhibition (%) |
|||
Replicate |
Mean value |
||||
1 |
2 |
3 |
|||
430 |
457 |
460 |
459 |
459 |
5.3 |
Control cultures |
487 |
481 |
484 |
484 |
0 |
491 |
477 |
starting value of the biomass in the control (t0): 8
Description of key information
Toxicity to microorganisms: not toxic to microorganisms: inhibition of growth < 10% (DIN 38412, part 8, Pseudomonas cell multiplication inhibition test) at a nominal loading rate of 430 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 430 mg/L
Additional information
The EC10 for the test material is much higher than its water solubility.
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