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Diss Factsheets
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EC number: 231-209-7 | CAS number: 7446-81-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro DNA damage and/or repair study
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 26 Feb 1987 - 15 Jan 1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
- Reference Type:
- publication
- Title:
- Genetic Toxicology of Acrylic Acid.
- Author:
- McCarthy KL et al.
- Year:
- 1 992
- Bibliographic source:
- Fd. Chem. Toxic. 30: 505-515
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 482 (Genetic Toxicology: DNA Damage and Repair, Unscheduled DNA Synthesis in Mammalian Cells In Vitro)
- GLP compliance:
- yes
- Type of assay:
- other: Unscheduled DNA synthesis (UDS)
Test material
- Reference substance name:
- Acrylic acid
- EC Number:
- 201-177-9
- EC Name:
- Acrylic acid
- Cas Number:
- 79-10-7
- Molecular formula:
- C3H4O2
- IUPAC Name:
- acrylic acid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Acrylic acid
- Analytical purity: > 99.8 %
- Source: Hoechst Celanese Company, Somerville, NJ, USA
Method
Species / strain
- Species / strain / cell type:
- hepatocytes: primary culture (rat)
- Details on mammalian cell type (if applicable):
- Primary rat liver cell cultures were derived from the livers of normal adult male Sprague-Dawley rats according to the procedure described by Williams et al. (In Vitro 13: 809-817, 1977):
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- 0.01, 0.03, 0.06, 0.1, 0.2, 0.3, 0.4, 0.6 µL/mL
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: phosphate buffered saline
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 7,12-dimethylbenzanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: 18-20 hrs (with test article and 3H-thymidine)
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED: 25 cells in random areas on each of two coverslips per treatment
DETERMINATION OF CYTOTOXICITY
- Method: lactic acid dehydrogenase (LDH) activity
- Evaluation criteria:
- If the mean net nuclear count was increased by at least five counts over the control, the results for a particular dose level were considered significant. A test article was judged positive if it induced a dose-related response and at least one dose produced a significant increase in the average net nuclear grains when compared to that of the control. In the absence of a dose-response, a test article which showed a significant increase in the mean net nuclear grain count in at least two successive doses was considered positive.
Results and discussion
Test results
- Key result
- Species / strain:
- hepatocytes: Primary Rat Hepatocytes
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 0.4 µL/mL
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- True negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
The test article, acrylic acid, was tested in the Unscheduled DNA Synthesis Test using rat primary hepatocytes. The test article was originally tested at nine dose levels ranging from 0.001 to 3.0 µL/mL and was fully evaluated at five dose levels of 0.01, 0.03, 0.1, 0.2 and 0.3 µL/mL. A repeat assay was conducted using eight dose levels ranging from 0.01 to 0.6 µL/mL and was fully evaluated at seven dose levels of 0.01, 0.03, 0.06, 0.1, 0.2, 0.3 and 0.4 µL/mL. The results of the original UDS assay indicate that under the test conditions, the test article did cause a significant increase in the mean number of net nuclear grain counts (i.e., an increase of at least 5 counts over the control), at the second highest dose level evaluated, 0.2 µL/mL. The remaining dose levels showed no increase in net nuclear counts above the control. Since an analysis of the raw data indicated that there had not been an actual increase in nuclear counts, only a decrease in cytoplasmic counts, a repeat assay was performed. In the repeat assay the test article did not cause a significant increase in mean net nuclear grain counts at any dose level. Therefore, the test article was considered to be negative in this study.
Summary of Repeat UDS Assay:
Treatment [µL/mL] |
Relative survival [%] |
Average net grains/nucleus |
% cells with 5 or more net nuclear grains |
Acrylic acid 0.6 |
26 |
- |
- |
Acrylic acid 0.4 |
90 |
0.0 ± 1.6 |
0 |
Acrylic acid 0.3 |
94 |
-0.4± 1.6 |
0 |
Acrylic acid 0.2 |
96 |
0.1 ± 1.4 |
0 |
Acrylic acid 0.1 |
96 |
0.1± 0.9 |
0 |
Acrylic acid 0.06 |
97 |
0.1 ± 1.6 |
2 |
Acrylic acid 0.03 |
99 |
0.1 ± 1.0 |
0 |
Acrylic acid 0.01 |
100 |
-0.6 ± 1.9 |
0 |
DMBA 10 µg/mL |
79 |
12.6 ± 4.8 |
100 |
DMBA 3 µg/mL |
88 |
6.2 ± 3.2 |
66 |
DMSO |
100 |
-0.7 ± 1.5 |
0 |
PBS |
100 |
0.1 ±1.0 |
0 |
Media control |
98 |
-0.2± 1.4 |
0 |
DMBA: 7,12- Dimethylbenzanthracene (positive control)
DMSO:Dimethylsulfoxide (vehicle control to positive control)
PBS: Phosphate buffered saline (vehicle control)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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