Dinitrotoluene

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1992

Report date:

1975

Reference 2

Reference Type:

secondary source

Title:

Dinitrotoluene (isomers mixture), CAS No.: 25321-14-6

Author:

OECD SIDS

Year:

2004

Bibliographic source:

UNEP Publication

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

Principles of method if other than guideline:

The test was conducted in accordance with "Bioaccumulation test of chemical substance in fish and shellfish" stipulated in the Order Prescribing the Items of the Test Relating to the New Chemical Substance (1974, Order of the Prime Minister, the Minister of Health and Welfare, the Minister of International Trade and Industry No. 1). This guideline corresponds to "305C, Bioaccumulation: Degree of Bioconcentration in Fish" stipulate in the OECD Guidelines for Testing of Chemicals (1981)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Dinitrotoluene

EC Number:

246-836-1

EC Name:

Dinitrotoluene

Cas Number:

25321-14-6

Molecular formula:

C7H6N2O4

IUPAC Name:

Reaction mass of 1-methyl-2,4-dinitrobenzene and 2-methyl-1,3-dinitrobenzene

Details on test material:

- Name of test material (as cited in study report): 2,4-/2,6-DNT (technical mixture)
- Analytical purity: no data

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Source: Sugishama fish farm
- Fish at start of incubation: ca. 30 g, ca. 10 cm
- After external desinfection under static conditions with 50 mg/l Terramycin and 7 g/l sodium chloride, the fish were reared in a flow through system for about 28 d
- Feeding during test: Fish feeding with pelleted food (Japan Haigo Shiryo K.K.), about 1 % of body weight twice per day

ACCLIMATION
- Fish were reared in an acclimatization tank (flow through system) for another 28 d at 25 +/- 2 °C
ion period:

Study design

Route of exposure:

aqueous

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

56 d

Test conditions

Test temperature:

25°+/- 1°C

Dissolved oxygen:

6-8 mg/L

Details on test conditions:

- Incubation of each 15-20 fish per level in glass tank containing 100 l of liquid each
- Water was groundwater from the Kurume Research Laboratories
- Water temperature, pH, dissolved oxygen were continuously measured
- Total hardness, COD, chloride, and other parameters were measured every 6 months

Nominal and measured concentrations:

0.025 mg/L

Results and discussion

Lipid content

Lipid content:

6.8 %

Time point:

start of exposure

Bioaccumulation factor

Key result

Type:

BCF

Value:

> 3.2 - < 21.2 dimensionless

Any other information on results incl. tables

With a concentration of 0.25 mg/L, a BCF of 0.6 - 2.9 was obtained.