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EC number: 210-817-6 | CAS number: 623-84-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 January - 21 February 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to test guidelines and in accordance with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Propane-1,2-diyl diacetate
- EC Number:
- 210-817-6
- EC Name:
- Propane-1,2-diyl diacetate
- Cas Number:
- 623-84-7
- Molecular formula:
- C7H12O4
- IUPAC Name:
- propane-1,2-diyl diacetate
- Details on test material:
- Purity >99.5%
Constituent 1
Method
- Target gene:
- Strain Histidine mutation Mutation type
TA1537 his C3076 Frameshift
TA1538 his D3052 Frameshift
TA98 his D3052/R-factor* Frameshift
TA1535 his G46 Base-pair substitutions
TA100 his G46/R- factor * Base-pair substitutions
*R-factor = plasmid pKM101 (increases error-prone DNA repair).
Each tester strain contains the following additional mutations:
rfa: deep rough (defective lipopolysaccharide cell coat).
gal: mutation in the galactose metabolism.
chl: mutation in nitrate reductase.
bio: defective biotin synthesis.
uvrB: loss of the excision repair system (deletion of the ultraviolet-repair B gene).
Species / strain
- Species / strain / cell type:
- other: Strains TA98, TA100, TA1535, TA1537 and TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 from Aroclor 1254 induced male Wistar rat liver
- Test concentrations with justification for top dose:
- 100, 333, 1000, 3333 and 5000 ug/plate without S9
100, 333, 1000, 3333 and 5000 ug/plate with S9 - Vehicle / solvent:
- reagent water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- reagent water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: Without S9: TA1535-sodium azide, TA100-methylmethanesulfonate, TA98 and TA1538-4-nitro-o-phenylenediamine, TA1537-9aminoacridine; With S9: all strains-2-aminoanthracene
- Details on test system and experimental conditions:
- The Salmonella tester strains have been provided by Dr. Bruce N. Ames, University of California at Berkeley, USA.
Samples of frozen stock cultures of bacteria are transferred into enriched nutrient broth (Oxoid No.2) and incubated in a shaking water bath (37C, 150 spm) until the cultures reach an 0.D. of 0.4 at 700 nm ( 10(9) cells/ml). Freshly grown cultures of each strain are used for a test.
Standard plate test
Top agar in top agar tubes is melted and heated to 45°C. The following solutions are successively added to 3 ml of top agar: 0.1 ml of a fresh bacterial culture (10(9) cells/ml) of one of the tester strains, 0.1 ml of a dilution of the test substance in reagent grade water, and in the case of activation assays 0.5 ml of S9-mix. The ingredients are mixed on a Vortex and the contents of the top agar tube are poured onto a selective agar plate. After solidification of the top agar, the plates are.turned and incubated in the dark at 37°C for 48h. After this period revertant colonies (histidin independent) are counted automatically with an Artek model 880 colony counter or manually. - Evaluation criteria:
- No additional information available.
- Statistics:
- No additional information available.
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537 and TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- In a preliminary test with strain TA100, eleven serial dilutions of the test substance, in approximately half-log steps, were plated with a diluted TA100 culture on non-selective agar (viability counting). For viability determinations, equal numbers of bacterial cells were seeded on each plate in the presence of the test substance. The percentage survival of an appropriately diluted TA100 culture on non-selective agar is determined by comparing the number of colonies on the solvent control plate with those on the plates containing the test substance. However, even at the highest test substance
concentration used the survival of strain TA100 is not reduced. Based on these data, the test substance was tested up to a concentration of 5 mg/plate, which is about the maximum test substance concentration that should be used, according to the OECD guidelines.
The Ames Salmonella microsome plate test
All bacteri al strains showed negative responses over the entire dose range of the test substance, i. e. no statistically significant dose-related increase in the number of revertants. Strain-specific positive control chemicals showed that the test conditions were optimal and that the metabolic activation system functioned properly. Based on these results, the test substance can be considered as nonmutagenic in the Ames Salmonella/microsome assay. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1 MUTAGENIC RESPONSE OF 1,2 DIACETOXY-PROPANE IN THE AMES SALMONELLA/MICROSOME
PLATE TEST
Experiment 1
Mean number of revertant (His+) colonies/3 replicate plates (+S.D) with different strains of S. typhimurium | |||||
Dose (ug/plate) | TA1535 | TA1537 | TA1538 | TA98 | TA100 |
Without S9 -mix | |||||
100 | 11 + 3 | 11 + 3 | 18 + 4 | 39 + 2 | 105 + 14 |
333 | 12 + 1 | 9 + 3 | 18 + 4 | 48 + 3 | 115 + 11 |
1000 | 11 + 6 | 13 + 4 | 21 + 4 | 46 + 4 | 129 + 13 |
3333 | 12 + 1 | 11 + 1 | 17 + 4 | 48 + 3 | 118 + 17 |
5000 | 13 + 6 | 12 + 1 | 18 + 2 | 43 + 6 | 111 + 3 |
Solvent controla) | 10 + 5 | 13 + 1 | 19 + 2 | 39 + 3 | 118 + 8 |
Positive control | 275 + 33 | 1410 + 125 | 1985 + 108 | 2365 + 192 | 1207 + 74 |
With S9 -mix | |||||
100 | 13 + 6 | 10 + 3 | 28 + 2 | 43 + 5 | 111 + 7 |
333 | 11 + 2 | 12 + 5 | 30 + 3 | 38 + 5 | 122 + 2 |
1000 | 10 + 4 | 11 + 3 | 25 + 2 | 39 + 10 | 126 + 2 |
3333 | 12 + 2 | 12 + 3 | 26 + 6 | 40 + 5 | 124 + 6 |
5000 | 10 + 1 | 12 + 4 | 26 + 6 | 34 + 5 | 125 + 5 |
Solvent controla) | 11 + 3 | 11 + 6 | 27 + 4 | 43 + 4 | 121 + 2 |
Positive control | 169 + 3 | 123 + 26 | 833 + 45 | 1330 + 27 | 1887 + 86 |
a) 0.1 ml reagent grade water.
Table 2 MUTAGENIC RESPONSE OF 1,2 DIACETOXY-PROPANE IN THE AMES SALMONELLA/MICROSOME
PLATE TEST
Experiment 2
Mean number of revertant (His+) colonies/3 replicate plates (+S.D) with different strains of S. typhimurium | |||||
Dose (ug/plate | TA1535 | TA1537 | TA1538 | TA98 | TA100 |
100 | 12 + 5 | 18 + 2 | 19 + 3 | 32 + 5 | 168 + 64 |
333 | 10 + 3 | 15 + 6 | 13 + 3 | 30 + 6 | 105 + 12 |
1000 | 12 + 6 | 19 + 5 | 22 + 4 | 32 + 8 | 110 + 24 |
3333 | 9 + 3 | 22 + 3 | 13 + 3 | 35 + 5 | 116 + 13 |
5000 | 9 + 1 | 14 + 4 | 17 + 5 | 28 + 3 | 138 + 30 |
Solvent controla) | 8 + 4 | 15 + 5 | 16 + 5 | 31 + 5 | 109 + 9 |
Positive control | 264 + 55 | 1678 + 85 | 1237 + 35 | 1198 + 87 | 773 + 41 |
With S9-mix | |||||
100 | 10 + 1 | 17 + 3 | 19 + 1 | 28 + 5 | 111 + 26 |
333 | 7 + 2 | 15 + 4 | 21 + 6 | 28 + 5 | 108 + 16 |
1000 | 9 + 1 | 16 + 5 | 18 + 2 | 36 + 8 | 123 + 4 |
3333 | 11 + 2 | 13 + 2 | 24 + 2 | 33 + 7 | 124 + 14 |
5000 | 12 + 2 | 15 + 3 | 22 + 7 | 36 + 9 | 108 + 6 |
Solvent controla) | 11 + 1 | 16 + 1 | 16 + 2 | 32 + 5 | b) |
Positive control | 141 + 8 | 69 + 10 | 873 + 94 | 1111 + 168 | 1999 + 52 |
a) 0.1 ml reagent grade water.
b) Inadvertently not measured
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test substance induced no statistically significant dose related increase in the numbers of revertant (His+) colonies in any of the five tester strains (TA 1535; TA 1537; TA 1538; TA 98; TA 100) with or without metabolic activation. The test substance can therefore be considered as non mutagenic in this test system. - Executive summary:
PGDA (propylene glycol diacetate) was tested in the Ames Salmonella/microsome test up to the limit of toxicity (5 mg/plate).
The test substance induced no statistically significant dose related increase in the numbers of revertant (His+) colonies in any of the five tester strains (TA 1535; TA 1537; TA 1538; TA 98; TA 100). The test substance can therefore be considered as non mutagenic in this test system.
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