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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.

Data source

Reference
Reference Type:
publication
Title:
Acute toxicity tests on 113 environmental chemicals.
Author:
Hasegawa R, Nakaji Y, Kurokawa Y and Tobe M.
Year:
1989
Bibliographic source:
Sci. Rep. Res. Inst. Tohoku Univ. C 36: 1-4 and 10-16

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Mice were exposed to ETU by a single oral administration. The LD50 was determined in males and females.
GLP compliance:
not specified
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Imidazolidine-2-thione
EC Number:
202-506-9
EC Name:
Imidazolidine-2-thione
Cas Number:
96-45-7
Molecular formula:
C3H6N2S
IUPAC Name:
imidazolidine-2-thione
Details on test material:
Other name = Ethylenethiourea (ETU)
purity = "highest purity available"
ETU was purchased from various commercial source or was contributed by cooperating chemical companies.

Test animals

Species:
mouse
Strain:
other: ddY
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Shizuoka Laboratory Animal Center (Hamamatsu, Japan)
- Age at study initiation: 5 weeks old
- Weight at study initiation: no data
- Fasting period before study: 15 hours before administration
- Housing: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: 1 week

ENVIRONMENTAL CONDITIONS : no data

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
olive oil
Details on oral exposure:
No data
Doses:
6 increasing dose levels were selected and tested to confirm both LDO and LD100 values.
No. of animals per sex per dose:
10 animals/dose
Control animals:
not specified
Details on study design:
Clinicals signs and symptoms were monitored during the experimental period. animals were given regular laboratory chow 6 hr after a single administration and the number of dead animals were counted until the 14th day. Anatomical examination was performed immediately after death or at terminal sacrifice on day 14.
Statistics:
The fifty percent of lethal dose (LD50) was calculed using the Litchfield and Wilcoxon method.

Results and discussion

Preliminary study:
no data
Effect levelsopen allclose all
Sex:
female
Dose descriptor:
LD50
Effect level:
4 350 mg/kg bw
95% CL:
4 020 - 4 710
Sex:
male
Dose descriptor:
LD50
Effect level:
4 150 mg/kg bw
95% CL:
3 640 - 4 730
Mortality:
yes
Clinical signs:
other: Acute toxic symptoms observed were hypoactivity and tonic convulsion.
Gross pathology:
no data
Other findings:
Changes in tissues were observed : atrophy of spleen and thymus.

Applicant's summary and conclusion

Conclusions:
LD50 for rats (male/female) were 4125/4350 mg/kg/day in this study.
Executive summary:

Mice were exposed to ETU by a single oral administration. The LD50 was determined in males and females. LD50 for rats (male/female) were 4125/4350 mg/kg/day in this study.