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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Apears to be well conducted and reported sufficiently to make an assessment of the endpoint. Prior to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
method according to 16 CFR 1500.42.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Nitrilotriacetonitrile
EC Number:
230-804-9
EC Name:
Nitrilotriacetonitrile
Cas Number:
7327-60-8
Molecular formula:
C6H6N4
IUPAC Name:
2-[bis(cyanomethyl)amino]acetonitrile
Details on test material:
White Crystalline powder
Purity >99%

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
6 animals between 2 and 4 kg purchased from Dutchland Lab. Inc., Denver, Pennsylvania

Test system

Vehicle:
unchanged (no vehicle)

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: average of 24, 48 and 72
Score:
0.11
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24, 48, 72 hr mean
Score:
0
Max. score:
2
Reversibility:
other: no effects
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 24, 48, 72 hr mean
Score:
1.1
Max. score:
3
Reversibility:
fully reversible
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 24, 48, 72 hours mean
Score:
0
Max. score:
4
Reversibility:
other: no effects
Irritant / corrosive response data:
In all animals there was some conjunctival reddening that did not persist to 7 days. One animal had a slight corneal opacity that did not persist to 7 days. This substance is not considered irritating to the eyes.

Any other information on results incl. tables

  Cornea Iris Conjuctiva red Conjuctiva swelling
Animal 24 48 72 mean 24 48 72 mean 24 48 72 mean 24 48 72 mean
1 0 1 1 0.666667 0 0 0 0 2 1 0 1 0 0 0 0
2 0 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0
3 0 0 0 0 0 0 0 0 2 1 1 1.333333 0 0 0 0
4 0 0 0 0 0 0 0 0 2 2 0 1.333333 0 0 0 0
5 0 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0
6 0 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0
Mean 0.111111 0 1.111111 0

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The test material was not irritating to the eyes.