1,4-bis(p-tolylamino)anthraquinone

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two toxicity study of micro-organismsfor the test chemical :
1 . and 2. Tetrahymena pyriformis population growth impairment assay was carried out for 48 hr to study the effects of test chemical.
Toxicity to micro-organisms test was carried out according to the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

2.This solvent h a s low toxicity to Tetrahymena , low volatility, a n d high ability to dissolve most organic chemicals. Concentrations no greater that t 0.75% DMSO (37 5 m L per 5 0 mL of medium) are used.
3. Toxicity to micro-organisms test was carried out according to the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test.

Test organisms (species):

other: 2. Tetrahymena pyriformis and 3.activated sludge

Details on inoculum:

2. - Strain: GL
- Source: John R. Kennedy, Department of Biochemistry, Cell and Molecular Biology, The University of Tennessee -Knoxville

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Remarks on exposure duration:

2. 3 hr

Test temperature:

2) 2 7 +- 1°C

Details on test conditions:

2. TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): foam -stoppered closed
- Material, size, headspace, fill volume: 2 5 0 -m L

Duration:

48 h

Dose descriptor:

other: IGC50

Effect conc.:

0.228 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Conclusions:

The test chemical effect concentration value is in the range of 0.228 to >100 mg/L.

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:

For structurally similar read across substance , tetrahymena pyriformis population growth impairment assay was carried out for 48 hr to study the effects of test chemical on micro-organisms. The population growth inhibition IGC50 to 50% of Tetrahymena pyriformis when exposed to test chemical for 48 hr under static condition is 0.228 mg/L.

In a data source for another read across substance toxicity to micro-organisms test was carried out according to the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test.The 50% inhibition IC50 of micro-organsms when exposed to test chemical is >100 mg/L. The test chemical test material effect concentration value is in the range of 0.228 to >100 mg/L.

Description of key information

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:

For structurally similar read across substance , tetrahymena pyriformis population growth impairment assay was carried out for 48 hr to study the effects of test chemical on micro-organisms. The population growth inhibition IGC50 to 50% of Tetrahymena pyriformis when exposed to test chemical for 48 hr under static condition is 0.228 mg/L.

In a data source for another read across substance toxicity to micro-organisms test was carried out according to the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test.The 50% inhibition IC50 of micro-organsms when exposed to test chemical is >100 mg/L. The test chemical test material effect concentration value is in the range of 0.228 to >100 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:

100 mg/L

Additional information

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of microorganism of the test chemical .The studies are as mentioned below:

For structurally similar read across substance , tetrahymena pyriformis population growth impairment assay was carried out for 48 hr to study the effects of test chemical on micro-organisms. The population growth inhibition IGC50 to 50% of Tetrahymena pyriformis when exposed to test chemical for 48 hr under static condition is 0.228 mg/L.

In a data source for another read across substance toxicity to micro-organisms test was carried out according to the OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test.The 50% inhibition IC50 of micro-organsms when exposed to test chemical is >100 mg/L. The test chemical test material effect concentration value is in the range of 0.228 to >100 mg/L.