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Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From December 21, 1992 to January 15, 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
Deionised water instead of distilled water used for preparation of stock solution; preparation of the inoculum: higher sludge weight after the washing procedure (sludge concentration in the test according to the guidelines); 4 instead of 5 test conc
Qualifier:
according to guideline
Guideline:
other: EG Method L 113/118
Principles of method if other than guideline:
Guidelines followed
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
None
Analytical monitoring:
no
Details on sampling:
No data
Vehicle:
not specified
Details on test solutions:
No data
Test organisms (species):
activated sludge, domestic
Details on inoculum:
Activated sludge from ARA Pro Rheno Basel (communal sewage treatment plant).
Pretreated by washing 3 times with tap water and addition of synthetic sewage (one day before main test).
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
No data
Hardness:
No data
Test temperature:
20.7 °C
pH:
No data
Dissolved oxygen:
No data
Salinity:
No data
Nominal and measured concentrations:
No data
Details on test conditions:
No data
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Key result
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
None
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Relevant effect levels: 3h IC50 = 12.5 mg/L
Reported statistics and error estimates:
No data

None

Validity criteria fulfilled:
yes
Conclusions:
3 h IC50 to activated sludge was >320 mg/L.
Executive summary:

A study was conducted to determine the toxicity of the test substance (of ca. 100% purity) to activated sludge according to OECD 209 and EG Method L 133/18.

Sludge from a domestic wastewater treatment plant was exposed for 3 h at 0, 10, 32, 100 and 320 mg/L. The reference substance 3 -5, dichlorophenol was also tested at 3.2, 10 and 32 mg/L.

Based on the findings of the study, the 3 h IC50 was >320 mg/L. The 3 h IC50 for the reference substance was 12.5 mg/L.

Description of key information

3-hour IC50 of FAT 40171/D  to activated sludge micro-organisms was determined to be >320 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
320 mg/L

Additional information

The key study was performed to evaluate the inhibitory effect of the test item FAT 40171/D on the respiration rate of aerobic wastewater micro-organisms of activated sludge in a 3-hour respiration inhibition test according to the OECD Guideline for Testing of Chemicals, No. 209.

Thus, the 3-hour IC50 of FAT 40171 to activated sludge micro-organisms was determined to be >320 mg/L.

Several other studies performed between 1983 to 1993 reported 3-hour IC50 to be >320 mg/L.