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EC number: 275-108-6 | CAS number: 71002-20-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 008
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- The algal growth inhibition test was modified from Dutka & Bitton (1986), Toxicity Testing Using Microorganisms, Vol. 2, CRC Press, Inc, Florida, pp. 79-100. Total exposure duration: 96 h
- Principles of method if other than guideline:
- The algal growth inhibition test was modified from Dutka & Bitton (1986), Toxicity Testing Using Microorganisms, Vol. 2, CRC Press, Inc, Florida, pp. 79-100.
Total exposure duration: 96 h - GLP compliance:
- no
Test material
- Reference substance name:
- Octasodium 2,2'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino(1-hydroxy-3,6-disulphonatonaphthalene-2,8-diyl)azo]]bisnaphthalene-1,5-disulphonate
- EC Number:
- 275-108-6
- EC Name:
- Octasodium 2,2'-[1,4-phenylenebis[imino(6-chloro-1,3,5-triazine-4,2-diyl)imino(1-hydroxy-3,6-disulphonatonaphthalene-2,8-diyl)azo]]bisnaphthalene-1,5-disulphonate
- Cas Number:
- 71002-20-5
- Molecular formula:
- C52H26Cl2N14Na8O26S8
- IUPAC Name:
- octasodium 3-({4-chloro-6-[(4-{[4-chloro-6-({8-[(1E)-2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-1-hydroxy-3,6-disulfonatonaphthalen-2-yl}amino)-1,3,5-triazin-2-yl]amino}phenyl)amino]-1,3,5-triazin-2-yl}amino)-5-[(1E)-2-(1,5-disulfonatonaphthalen-2-yl)diazen-1-yl]-4-hydroxynaphthalene-2,7-disulfonate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- TEST WATER:
The test water adapted from Dutka & Bitton (1986) was used as a culture medium for dye toxicity testing to algae.
Test organisms
- Test organisms (species):
- Chlorella sp.
- Details on test organisms:
- The Chlorella sp. was obtained from cultured maintained at the Thailand Institute of Scientific and Technical Research. It was grown in 5 L glass jars containing growth medium to obtain the stock algal culture to be used for all experiments.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 4 d
Test conditions
- Test temperature:
- ambient temperature
- Nominal and measured concentrations:
- nominal concentrations: 50, 100, 150, 200, 250 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- humic acid
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 95.55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Any other information on results incl. tables
When consider the absorbance of Reactive Red 141, it is evident that Reactive Red 141 give the maximum magnitude of wavelength 544 nm. Hence, it could affect the light transmitted in the water, ultimately affecting the light absorption of Chlorophyll a in photosynthesis.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In this toxicity test to algae (Chlorella sp.) an 96 hr-EC 50 value of 95.55 mg/L was obtained for Reactive Red 141.
- Executive summary:
The algal growth inhibition test was modified from Dutka & Bitton (1986) and performed in 500 mL Erlenmeyer flask.
Algae were added into 100 mL of test water containing various concentrations of Reactive Red 141 (final concentration of 1 x 105 cells/mL). The flask was placed in an ambient temperature provided air and sealed with parafilm. Flasks were continuous illuminated (cool white fluorescent light approximately 120 ~J.E/s/m2 or 6,000 lux at water level. Determination of algal growth by measuring live cell count, Chlorophyll a, dry weight and absorbance at 720 nm (this wavelength is not interfered by the dyes) was done. The ECso for dyes toxicity to algae was estimated. The toxicity to algae was reported in term of 96-h EC50 which referred to the concentration of dye that inhibit the the growth of Chlorella sp. in 50% of the population after 96 h exposure. An 96 hr-EC 50 value of 95.55 mg/L was obtained for Reactive Red 141.
As Reactive Red 141 is a coloured substance, the effect of light absorption at standard algae growth inhibition tests has to be considered. Absorption of light at relevant wavelengths may cause an indirect effect on aquatic plant growth by inhibition of photosynthesis.
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