Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2007

Report date:

2007

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

EC Number:

262-475-2

EC Name:

Tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran

Cas Number:

60857-05-8

Molecular formula:

C10H16O

IUPAC Name:

4-methylidene-2-(2-methylprop-1-en-1-yl)oxane

Details on test material:

- Chemical name of test material: tetrahydro-4-methylene-2-(2-methyl-1-propenyl)-2H-pyran
- Lot/batch No.: LJ 1-28/29
- Analytical purity: 99.2 area %

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test concentrations were prepared separately by adding the required amounts test substance to 180 ml test water and stirring the mixture for 20 ± 2 hours at room temperature.

Test organisms

Test organisms (species):

activated sludge, domestic

Details on inoculum:

Activated sludge taken from a municipal waste water treatment plant
- Initial biomass concentration: 1.5 g/l dry substance

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

180 min

Test conditions

Test temperature:

22 ± 2 °C

pH:

7.3 - 7.9

Nominal and measured concentrations:

Nominal test substance concentrations:
62.5, 125, 250, 500 and 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer-vessel (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 ml/vessel 100-fold concentrated OECD medium

Reference substance (positive control):

yes

Remarks:

3,5 dichlorphenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

180 min

Dose descriptor:

EC10

Effect conc.:

980 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

180 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

180 min

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate